A peptide export-import control circuit modulating bacterial development regulates protein phosphatases of the phosphorelay
A peptide export-import control circuit modulating bacterial development regulates protein phosphatases of the phosphorelay
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DOI：
10.1073/pnas.94.16.8612
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发表时间：
1997-08-05
影响因子：
11.1
通讯作者：
Perego, M
中科院分区：
文献类型：
--
作者：
Perego, M
磷酸化信号转导系统通过Spo 0 F和Spo 0A反应调节剂的乙酰基残基的磷酸化来激活枯草芽孢杆菌孢子形成中的发育转录。如果这些反应调节剂的磷酸化水平由蛋白激酶和蛋白天冬氨酸磷酸酶的相反活性确定，则这些蛋白激酶和蛋白天冬氨酸磷酸酶在信号整合电路中解释发育的正信号和负信号，磷酸化中继的RapA蛋白天冬氨酸磷酸酶由直接抑制其活性的肽调节，该肽由phrA基因中编码的无活性前抑制剂蛋白进行蛋白水解加工。前抑制剂被蛋白质输出装置切割成推定的前抑制剂，其被进一步加工成活性抑制剂肽并被寡肽通透酶内化。这种输出-输入回路被认为是一种定时磷酸酶活性的机制，其中加工酶调节活性抑制剂的形成速率。加工事件可能反过来受到一个调节层次的控制。染色体测序揭示了B中的其他几个磷酸酶-前肽基因对。枯草杆菌，这表明使用这种机制可能是广泛的信号转导。
The phosphorelay signal transduction system activates developmental transcription in sporulation of Bacillus subtilis by phosphorylation of aspartyl residues of the Spo0F and Spo0A response regulators, The phosphorylation level of these response regulators if determined by the opposing activities of protein kinases and protein aspartate phosphatases that interpret positive and negative signals for development in a signal integration circuit, The RapA protein aspartate phosphatase of the phosphorelay is regulated by a peptide that directly inhibits its activity, This peptide is proteolytically processed from an inactive pre-inhibitor protein encoded in the phrA gene. The pre-inhibitor is cleaved by the protein export apparatus to a putative pro-inhibitor that is further processed to the active inhibitor peptide and internalized by the oligopeptide permease. This export-import circuit is postulated to be a mechanism for timing phosphatase activity where the processing enzymes regulate the rate of formation of the active inhibitor, The processing events may, in turn, be controlled by a regulatory hierarchy, Chromosome sequencing has revealed several other phosphatase-prepeptide gene pairs in B. subtilis, suggesting that the use of this mechanism may be widespread in signal transduction.