Cytogenetic and flow cytometric DNA analysis in renal cell carcinoma.
Cytogenetic and flow cytometric DNA analysis in renal cell carcinoma.
复制标题
肾细胞癌的细胞遗传学和流式细胞术 DNA 分析。
作者：
B. Ljungberg;I. Nordenson;G. Roos
对 16 名肾细胞癌患者的 37 个肿瘤样本进行了细胞遗传学和流式细胞术 DNA 分析。 36 例培养物染色体异常，1 例正常。最常见的异常是7号染色体三体性或四体性（16个肿瘤中的13个），表明这种异常可能是肾细胞癌的原发性改变。 16 个肿瘤中有 9 个（56%）发现了 3 号染色体异常。多个肿瘤样本的细胞遗传学结果显示，11 个肿瘤中有 8 个（73%）显示肿瘤内细胞遗传学异质性。 16 名患者中有 12 名患有非整倍体肿瘤，4 名患有同质二倍体肿瘤。 DNA指数与染色体数目相关。未发现细胞遗传学结果与临床分期之间存在关联。
Cytogenetic and flow cytometric DNA analyses were performed on 37 tumor samples from 16 patients with renal cell carcinoma. 36 cultures were chromosomally abnormal and 1 was normal. The most frequent abnormality was trisomy or tetrasomy of chromosome 7 (13 of 16 tumors), suggesting that this abnormality might be a primary change in renal cell carcinoma. Abnormalities of chromosome 3 were seen in 9 of 16 tumors (56%). Eight of 11 tumors (73%) with cytogenetic results from multiple tumor samples showed intratumor cytogenetic heterogeneity. Twelve of the 16 patients had aneuploid tumors, and 4 had homogeneously diploid tumors. DNA index correlated with chromosome number. No association was found between cytogenetic results and clinical stage.