SARS E protein in phospholipid bilayers: an anomalous X-ray reflectivity study
SARS E protein in phospholipid bilayers: an anomalous X-ray reflectivity study
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DOI：
10.1016/j.physb.2004.11.015
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发表时间：
2005-02-28
影响因子：
2.8
通讯作者：
Salditt, T
中科院分区：
文献类型：
--
作者：
Khattari, Z;Brotons, G;Salditt, T
我们报告一个异常的X射线反射率的研究，以定位标记的残留物的膜蛋白相对于脂质双层。从这样的实验中，可以推导出对蛋白质或肽构象的重要限制。具体来说，我们的目标是本地化的SARS E蛋白，纳入DMPC磷脂双层，这是沉积在硅表面上的厚多层堆栈的形式的碘标记的苯丙氨酸。在这里，我们讨论的实验方面和困难与傅立叶合成分析，给出了电子密度分布的膜。(C)2004 Elsevier B.V.保留所有权利。
We report on an anomalous X-ray reflectivity study to locate a labelled residue of a membrane protein with respect to the lipid bilayer. From such experiments, important constraints on the protein or peptide conformation can be derived. Specifically, our aim is to localize an iodine-labelled phenylalanine in the SARS E protein, incorporated in DMPC phospholipid bilayers, which are deposited in the form of thick multilamellar stacks on silicon surfaces. Here, we discuss the experimental aspects and the difficulties associated with the Fourier synthesis analysis that gives the electron density profile of the membranes. (C) 2004 Elsevier B.V. All rights reserved.