RAPD and Internal Transcribed Spacer Sequence Analyses Reveal Zea nicaraguensis as a Section Luxuriantes Species Close to Zea luxurians
RAPD and Internal Transcribed Spacer Sequence Analyses Reveal Zea nicaraguensis as a Section Luxuriantes Species Close to Zea luxurians
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DOI：
10.1371/journal.pone.0016728
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发表时间：
2011-04
期刊：
影响因子：
3.7
通讯作者：
Pei-Shan Wang;Yanli Lu;Mingmin Zheng;T. Rong;Qilin Tang
中科院分区：
文献类型：
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作者：
Pei-Shan Wang;Yanli Lu;Mingmin Zheng;T. Rong;Qilin Tang
基于随机扩增多态性 DNA (RAPD) 标记和核核糖体 DNA 的内转录间隔区 (ITS) 序列，使用来自玉米物种的 14 个种质，确定了新发现的尼加拉瓜类蜀黍（具有耐涝性的 Zea nicaraguensis）的遗传关系。 RAPD分析显示，136个随机十聚体引物共产生5,303个片段，其中多态性条带占84.86%。基于RAPD的UPGMA分析表明，玉米属可分为Luxuriantes组（包括Zea diloperennis、Zea luxurians、Zea perennis和Zea nicaraguensis）和Zea组（包括Zea mays ssp）。墨西哥，Zea mays ssp。 parviglumis，玉米亚种。 Huehuetenangensis 和 Zea mays ssp。梅斯。 ITS序列分析显示，玉米14个类群的整个ITS区域的长度从597到605 bp不等。平均GC含量为67.8%。除了插入/缺失之外，整个 ITS 区域还记录了 78 个可变位点，其中 47 个位于 ITS1，5 个位于 5.8S，26 个位于 ITS2。采用邻接（NJ）和最大简约（MP）方法对这些类群的序列进行分析，构建系统发育树，选择 Tripsacum dactyloides L. 作为外群。从 ITS 序列推断出的玉米物种的系统发育关系与解析两个主要亚属进化枝的 RAPD 证据高度一致。 RAPD和ITS序列分析均表明，与其他类蜀黍和栽培玉米相比，Zea nicaraguensis与Zea luxurians的亲缘关系更为密切，应视为Zea luxuriantes种。
Genetic relationship of a newly discovered teosinte from Nicaragua, Zea nicaraguensis with waterlogging tolerance, was determined based on randomly amplified polymorphic DNA (RAPD) markers and the internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA using 14 accessions from Zea species. RAPD analysis showed that a total of 5,303 fragments were produced by 136 random decamer primers, of which 84.86% bands were polymorphic. RAPD-based UPGMA analysis demonstrated that the genus Zea can be divided into section Luxuriantes including Zea diploperennis, Zea luxurians, Zea perennis and Zea nicaraguensis, and section Zea including Zea mays ssp. mexicana, Zea mays ssp. parviglumis, Zea mays ssp. huehuetenangensis and Zea mays ssp. mays. ITS sequence analysis showed the lengths of the entire ITS region of the 14 taxa in Zea varied from 597 to 605 bp. The average GC content was 67.8%. In addition to the insertion/deletions, 78 variable sites were recorded in the total ITS region with 47 in ITS1, 5 in 5.8S, and 26 in ITS2. Sequences of these taxa were analyzed with neighbor-joining (NJ) and maximum parsimony (MP) methods to construct the phylogenetic trees, selecting Tripsacum dactyloides L. as the outgroup. The phylogenetic relationships of Zea species inferred from the ITS sequences are highly concordant with the RAPD evidence that resolved two major subgenus clades. Both RAPD and ITS sequence analyses indicate that Zea nicaraguensis is more closely related to Zea luxurians than the other teosintes and cultivated maize, which should be regarded as a section Luxuriantes species.