Phylogenetic analysis based on 28S rRNA of Babesia spp. in ruminants in China
Phylogenetic analysis based on 28S rRNA of Babesia spp. in ruminants in China
复制标题
基于巴贝虫属28S rRNA的系统发育分析。
DOI：
10.1007/s10493-012-9607-0
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发表时间：
2013-04-01
影响因子：
2.2
通讯作者：
Luo, Jianxun
中科院分区：
文献类型：
--
作者：
Gou, Huitian;Guan, Guiquan;Luo, Jianxun
分子系统发育分析主要基于小核糖体RNA亚基（18S rRNA），内部转录间隔区和其他分子标记。我们比较了Babelusspp的系统发育关系。使用大亚基核糖体RNA，即，28S rRNA和28S + 18S rRNA联合序列片段。在中国收集。28 S rRNA基因序列较18 S rRNA基因包含更多的信息，可用于阐明B的亲缘关系。莫塔西湾，B.少校和B。牛因此，28S rRNA是另一个候选标记，可用于Babelands spp的系统发育分析。然而，联合片段（28S + 18S）分析提供了更好的支持系统发育关系比单一基因的Babyspp。在中国
Molecular phylogenetic analyses are mainly based on the small ribosomal RNA subunit (18S rRNA), internal transcribed spacer regions, and other molecular markers. We compared the phylogenetic relationships of Babesia spp. using large subunit ribosomal RNA, i.e., 28S rRNA, and the united 28S + 18S rRNA sequence fragments from 11 isolates of Babesia spp. collected in China. Due to sequence length and variability, the 28S rRNA gene contained more information than the 18S rRNA gene and could be used to elucidate the phlyogenetic relationships of B. motasi, B. major, and B. bovis. Thus, 28S rRNA is another candidate marker that can be used for the phylogenetic analysis of Babesia spp. However, the united fragment (28S + 18S) analysis provided better supported phylogenetic relationships than single genes for Babesia spp. in China.