Real-time imaging and quantitative analysis of doxorubicin transport in a perfusable microvessel platform
Real-time imaging and quantitative analysis of doxorubicin transport in a perfusable microvessel platform
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DOI：
10.1039/c6ib00082g
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发表时间：
2016-01-01
影响因子：
2.5
通讯作者：
Searson, Peter C.
中科院分区：
文献类型：
--
作者：
Bogorad, Max I.;Searson, Peter C.
在这里，我们报告的实时成像和定量分析的溶质运输灌注圆柱形微血管形成的Madin-Darby犬肾（MDCK）细胞包埋在胶原蛋白基质。使用荧光显微镜对注射后阿霉素转运的动力学进行成像。为了评估外排泵对转运的作用，在由MDCK形成的微血管中进行实验。2、MDCKII-w/t和MDCKII-MDR 1细胞。MDCKII-w/t和MDCKII-MDR 1显示多柔比星在细胞中显着积聚，这是多柔比星药代动力学的特征。我们提出了一个模型阿霉素运输，考虑到跨细胞层的运输。这些结果证明了细胞微血管的实时成像如何用于分析全身递送后的运输和分布机制。
Here we report on real-time imaging and quantitative analysis of solute transport in perfusable cylindrical microvessels formed from Madin-Darby canine kidney (MDCK) cells embedded in a collagen matrix. Fluorescence microscopy was used to image the kinetics of doxorubicin transport following injection. To assess the role of efflux pumps on transport, experiments were performed in microvessels formed from MDCK. 2, MDCKII-w/t, and MDCKII-MDR1 cells. MDCKII-w/t and MDCKII-MDR1 showed significant doxorubicin accumulation in the cells, characteristic of the pharmacokinetics of doxorubicin. We present a model for doxorubicin transport that takes into account transport across the cell layer. These results demonstrate how real-time imaging of cell microvessels can be used to analyze the mechanisms of transport and distribution following systemic delivery.