Genome-wide characterization and analysis of bZIP transcription factor gene family related to abiotic stress in cassava.
Genome-wide characterization and analysis of bZIP transcription factor gene family related to abiotic stress in cassava.
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木薯非生物胁迫相关bZIP转录因子基因家族的全基因组表征和分析
DOI：
10.1038/srep22783
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发表时间：
2016-03-07
影响因子：
4.6
通讯作者：
Li K
中科院分区：
文献类型：
--
作者：
Hu W;Yang H;Yan Y;Wei Y;Tie W;Ding Z;Zuo J;Peng M;Li K
碱性亮氨酸拉链（bZIP）转录因子家族在生物过程的各个方面起着至关重要的作用。目前，没有关于重要热带作物木薯中bZIP家族的信息。在此，从木薯中鉴定出77个bZIP基因。进化分析表明，MebZIPs可分为10个亚家族，保守基序和基因结构分析进一步支持了这一结论。全局表达分析表明，MebZIPs在栽培品种和野生亚种的不同组织中表现出相似或不同的表达模式。3个木薯基因型的转录组分析表明，许多MebZIP基因被激活的干旱在W14亚种的根，表明这些基因参与木薯的强大的抗旱性。选择的MebZIP基因在响应渗透，盐，冷，阿坝和H2O2的表达分析表明，他们可能参与不同的信号通路。我们对MebZIP基因的系统分析揭示了组成型、组织特异性和非生物胁迫响应的候选MebZIP基因，为进一步研究植物中MebZIP基因的功能提供了新的视角，为理解BZIP介导的非生物胁迫响应奠定了基础。
The basic leucine zipper (bZIP) transcription factor family plays crucial roles in various aspects of biological processes. Currently, no information is available regarding the bZIP family in the important tropical crop cassava. Herein, 77 bZIP genes were identified from cassava. Evolutionary analysis indicated that MebZIPs could be divided into 10 subfamilies, which was further supported by conserved motif and gene structure analyses. Global expression analysis suggested that MebZIPs showed similar or distinct expression patterns in different tissues between cultivated variety and wild subspecies. Transcriptome analysis of three cassava genotypes revealed that many MebZIP genes were activated by drought in the root of W14 subspecies, indicating the involvement of these genes in the strong resistance of cassava to drought. Expression analysis of selected MebZIP genes in response to osmotic, salt, cold, ABA, and H2O2 suggested that they might participate in distinct signaling pathways. Our systematic analysis of MebZIPs reveals constitutive, tissue-specific and abiotic stress-responsive candidate MebZIP genes for further functional characterization in planta, yields new insights into transcriptional regulation of MebZIP genes, and lays a foundation for understanding of bZIP-mediated abiotic stress response.