Cytotoxic T lymphocyte antigen-4 gene polymorphisms do not confer susceptibility to autoimmune hepatitis types 1 and 2 in Brazil
Cytotoxic T lymphocyte antigen-4 gene polymorphisms do not confer susceptibility to autoimmune hepatitis types 1 and 2 in Brazil
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DOI：
10.1016/s0002-9270(03)00307-1
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发表时间：
2003-07-01
影响因子：
9.8
通讯作者：
Goldberg, AC
中科院分区：
文献类型：
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作者：
Bittencourt, PL;Palácios, SA;Goldberg, AC
目的：不同人群对自身免疫性肝炎 (AIH) 的易感性与不同的 HLA-DR 抗原有关。最近，细胞毒性 T 淋巴细胞抗原 4 (CTLA-4) 基因外显子 1 的 A-G 多态性与北美白人的 1 型 AIH (AIH-1) 易感性相关。这种多态性与其他几种自身免疫性疾病有关，可能是因为它对 CTLA-4 的表达有影响，CTLA-4 是一种下调外周 T 细胞反应的粘附分子。本研究的目的是评估巴西 AIH-1 和 AIH 2 型 (AIH-1) 患者中 CTLA-4 基因型的频率，并探讨这些基因型对疾病表达的影响。 方法：通过聚合酶链反应对 106 名 AIH-1 患者、26 名 AIH-2 受试者和 67 名健康对照受试者进行 CTLA-4 基因型测定。 基于 (PCR) 的技术。结果：与健康对照相比，在 AIH-1 和 AIH-2 受试者中观察到 CTLA-4 基因型分布没有差异。 GG 基因型的 AIH-1 和 AIH-2 患者分别表现出较低的丙种球蛋白和 ALT 水平。结论：巴西患者对 AIH-1 和 AIH-2 的易感性不受第 49 位外显子 1 CTLA-4 基因多态性的影响。(C) 2003，Am。科尔。胃肠病学。
OBJECTIVES: Susceptibility to autoimmune hepatitis (AIH) has been linked to different HLA-DR antigens in distinct populations. Recently, an A-G polymorphism in exon 1 of the cytotoxic T lymphocyte antigen-4 (CTLA-4) gene was associated with predisposition to AIH type 1 (AIH-1) in white individuals in North America. This polymorphism has been associated with several other autoimmune diseases, presumably because of its effect in the expression of CTLA-4, an adhesion molecule that downregulates peripheral T cell responses. The aims of this study were to assess the frequency of CTLA-4 genotypes in Brazilian patients with AIH-1 and AIH type 2 (AIH-1), as well as to investigate the influence of these genotypes in disease expression.METHODS: Determination of CTLA-4 genotypes was carried out in 106 patients with AIH-1, 26 subjects with AIH-2, and 67 healthy control subjects by polymerase chain reaction (PCR)-based techniques.RESULTS: No difference in the distribution of CTLA-4 genotypes was observed in subjects with AIH-1 and AIH-2 as compared to healthy controls. Patients with AIH-1 and AIH-2 with the GG genotype exhibited lower gamma-globulin and ALT levels, respectively.CONCLUSIONS: Susceptibility to AIH-1 and AIH-2 in Brazilian patients is not influenced by exon 1 CTLA-4 gene polymorphisms at position 49. (C) 2003 by Am. Coll. of Gastroenterology.