Growth characteristics of human first trimester decidual cells cultured in serum-free medium: production of prolactin, prostaglandins and fibronectin.
Growth characteristics of human first trimester decidual cells cultured in serum-free medium: production of prolactin, prostaglandins and fibronectin.
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在无血清培养基中培养的人妊娠早期蜕膜细胞的生长特征：催乳素、前列腺素和纤连蛋白的产生。
DOI：
10.1095/biolreprod31.4.827
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发表时间：
1984
影响因子：
3.6
通讯作者：
Vlodavsky,I
中科院分区：
文献类型：
--
作者：
Hochner-Celnikier,D;Ron,M;Eldor,A;Segal,S;Palti,Z;Fuks,Z;Vlodavsky,I
在原代培养中建立纯的人早孕期蜕膜细胞的程序已经开发。获得这种培养物的高成功率是由于通过适当的酶解离和过滤初始组织样品来消除成纤维细胞，随后将细胞维持在补充有胰岛素、成纤维细胞生长因子（FGF）、雌二醇、孕酮、氢化可的松、转铁蛋白和亚硒酸钠的无血清培养基中。在这些培养条件下，我们获得了纯的和积极分裂的蜕膜细胞形成紧密包装和非重叠的上皮样细胞单层覆盖超过75%的培养面积。培养的蜕膜细胞保留其在体内产生催乳素和各种前列腺素（PGs），主要是PGE2的能力。培养20天后，激素产生明显减少。通过间接免疫荧光染色检测到大量的纤维状表面纤维连接蛋白网络。催乳素和前列腺素的产生以及纤维连接蛋白的分泌可能在胚胎的着床和随后的生长中起作用。所描述的获得无成纤维细胞的蜕膜细胞单层的方法将促进对子宫内生命早期该组织的激素调节的研究。
A procedure for the establishment of pure human first trimester decidual cells in primary cultures has been developed. The high rate of success in obtaining such cultures resulted from the elimination of fibroblasts by appropriate enzyme dissociation and filtration of the initial tissue sample, and subsequent maintenance of the cells in a serum-free medium supplemented with insulin, fibroblast growth factor (FGF), estradiol, progesterone, hydrocortisone, transferrin and sodium selenite. Under these culture conditions, we obtained pure and actively dividing decidual cells forming tightly packed and nonoverlapping epithelioid cell monolayers covering more than 75% of the culture area. The cultured decidual cells retained their in vivo capacity to produce prolactin and various prostaglandins (PGs), primarily PGE2. There was a marked reduction in hormone production after 20 days in culture. A massive network of fibrillar surface fibronectin was detected by indirect immunofluorescence staining of the cultured cells. The production of prolactin and PGs together with the secretion of fibronectin may play a role in the implantation and subsequent growth of the embryo. The described procedure of obtaining fibroblast-free decidual cell monolayers will promote studies on the hormonal regulation of this tissue at the time of early intrauterine life.