Microbial communities at the borehole observatory on the Costa Rica Rift flank (Ocean Drilling Program Hole 896A).
Microbial communities at the borehole observatory on the Costa Rica Rift flank (Ocean Drilling Program Hole 896A).
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DOI：
10.3389/fmicb.2012.00232
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发表时间：
2012
影响因子：
5.2
通讯作者：
Teske A
中科院分区：
文献类型：
--
作者：
Nigro LM;Harris K;Orcutt BN;Hyde A;Clayton-Luce S;Becker K;Teske A
由于难以进入地下环境，对洋中脊两侧受热液影响的地下玄武岩地壳的微生物学仍未得到充分研究。科学海洋钻探产生的仪器化钻孔可以获取流经洋壳的地层流体样本。我们分析了从海洋钻探计划 896A 号孔观测站现场收集的液体和微生物垫样本的细菌群落的系统发育多样性，该孔钻入赤道太平洋哥斯达黎加裂谷侧翼约 650 万年前的玄武岩地壳。从钻孔流体和覆盖流体端口外表面的微生物垫中回收的细菌 16S rRNA 基因序列揭示了独特和共有的系统发育型。两个样本中的优势细菌克隆均与自养、硫氧化硫微螺菌属相关。两个样本都产生了不同的γ-和α-变形菌系统型，以及拟杆菌门、浮霉菌门和疣微菌门的成员。对采样口垫和钻孔液体中的核酮糖 1,5-二磷酸羧化酶/加氧酶 (RuBisCO) 基因（cbbL 和 cbbM）进行的分析表明，原位微生物群落具有自养碳同化潜力；大多数cbbL基因与硫氧化属Thioalkalivibrio和Thiomicrospira相关，而cbbM基因与来自热液喷口羽流和海洋沉积物的未培养系统型有关。 896A 观测站的几个 16S rRNA 基因系统发育型与从暴露于海水的玄武岩和非活跃热液喷口硫化物沉积物中回收的系统发育型分组，但与胡安德富卡山脊侧翼受热液影响的玄武岩钻孔 1026B 和 U1301A 几乎没有重叠，这表明 896A 孔的位点特异性特征（即海水混合到钻孔流体中）会影响微生物群落的组成。
The microbiology of subsurface, hydrothermally influenced basaltic crust flanking mid-ocean ridges has remained understudied, due to the difficulty in accessing the subsurface environment. The instrumented boreholes resulting from scientific ocean drilling offer access to samples of the formation fluids circulating through oceanic crust. We analyzed the phylogenetic diversity of bacterial communities of fluid and microbial mat samples collected in situ from the observatory at Ocean Drilling Program Hole 896A, drilled into ~6.5 million-year-old basaltic crust on the flank of the Costa Rica Rift in the equatorial Pacific Ocean. Bacterial 16S rRNA gene sequences recovered from borehole fluid and from a microbial mat coating the outer surface of the fluid port revealed both unique and shared phylotypes. The dominant bacterial clones from both samples were related to the autotrophic, sulfur-oxidizing genus Thiomicrospira. Both samples yielded diverse gamma- and alphaproteobacterial phylotypes, as well as members of the Bacteroidetes, Planctomycetes, and Verrucomicrobia. Analysis of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) genes (cbbL and cbbM) from the sampling port mat and from the borehole fluid demonstrated autotrophic carbon assimilation potential for in situ microbial communities; most cbbL genes were related to those of the sulfur-oxidizing genera Thioalkalivibrio and Thiomicrospira, and cbbM genes were affiliated with uncultured phylotypes from hydrothermal vent plumes and marine sediments. Several 16S rRNA gene phylotypes from the 896A observatory grouped with phylotypes recovered from seawater-exposed basalts and sulfide deposits at inactive hydrothermal vents, but there is little overlap with hydrothermally influenced basaltic boreholes 1026B and U1301A on the Juan de Fuca Ridge flank, suggesting that site-specific characteristics of Hole 896A (i.e., seawater mixing into borehole fluids) affect the microbial community composition.
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影响因子：
4.2
作者：
CAUMETTE, P
通讯作者：
CAUMETTE, P
影响因子：
56.9
作者：
D'Hondt, S;Jorgensen, BB;Acosta, JLS
通讯作者：
Acosta, JLS
影响因子：
5.3
作者：
Becker, K;Davis, EE;deMoustier, CP
通讯作者：
deMoustier, CP
DOI：
10.1099/00207713-48-3-957
发表时间：
1998-07-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
Guyoneaud, R;Süling, J;Imhoff, JF
通讯作者：
Imhoff, JF
DOI：
10.1073/pnas.0811793106
发表时间：
2009-07-14
影响因子：
11.1
作者：
D'Hondt, Steven;Spivack, Arthur J.;Stancin, Andrea M.
通讯作者：
Stancin, Andrea M.