Combined effects of hyperaminoacidemia and oxandrolone on skeletal muscle protein synthesis
Combined effects of hyperaminoacidemia and oxandrolone on skeletal muscle protein synthesis
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DOI：
10.1152/ajpendo.2000.278.2.e273
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发表时间：
2000-02-01
影响因子：
5.1
通讯作者：
Ferrando, AA
中科院分区：
文献类型：
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作者：
Sheffield-Moore, M;Wolfe, RR;Ferrando, AA
我们研究了氧雄酮(Oxandrin，Ox)诱导的合成代谢在5天后是否维持了急性高氨基酸血症的正常合成作用。5名健康男性[22+/-3(SD)Yr]在口服牛(15 mg/d)5天前后进行了研究。在每项研究中，在5小时的基础期之后，再进行3小时的预充-持续输注商业氨基酸混合物(10%曲瓦索)。血液和肌肉样本的稳定同位素数据使用三室模型进行分析，以计算肌肉蛋白质的合成和分解。在对照组[基础对照(BC)vs对照+氨基酸(C+AA)；P<；0.001]和Ox研究[基础Ox(Box)vs Ox+氨基酸(Ox+AA)；P<；0.001]输入氨基酸后，模型来源的肌肉蛋白质合成增加，但蛋白质分解没有变化。肌肉蛋白质组分合成率分别提高94%(BC vs.C+AA；P=0.01)和53%(BOX vs.Ox+AA；P<；0.01)。我们的结论是，急性高氨基酸血症的正常合成代谢效应在接受氧雄酮诱导的合成代谢的骨骼肌中保持不变。
We investigated whether the normal anabolic effects of acute hyperaminoacidemia were maintained after 5 days of oxandrolone (Oxandrin, Ox)-induced anabolism. Five healthy men [22 +/- 3 (SD) yr] were studied before and after 5 days of oral Ox (15 mg/day). In each study, a 5-h basal period was followed by a 3-h primed-continuous infusion of a commercial amino acid mixture (10% Travasol). Stable isotopic data from blood and muscle sampling were analyzed using a three-compartment model to calculate muscle protein synthesis and breakdown. Model-derived muscle protein synthesis increased after amino acid infusion in both the control [basal control(BC) vs, control + amino acids (C+AA); P < 0.001] and Ox study [basal Ox (BOx) vs. Ox + amino acids (Ox + AA); P < 0.01], whereas protein breakdown was unchanged. Fractional synthetic rates of muscle protein increased 94% (BC vs. C+AA; P = 0.01) and 53% (BOx vs. Ox + AA; P < 0.01), respectively. We conclude that the normal anabolic effects of acute hyperaminoacidemia are maintained in skeletal muscle undergoing oxandrolone-induced anabolism.