Changes in Saccharomycescerevisiae Development Induced by Magnetic Fields
Changes in Saccharomycescerevisiae Development Induced by Magnetic Fields
复制标题
磁场引起的酿酒酵母发育变化
DOI：
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
F. R. Silva
中科院分区：
文献类型：
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作者：
M. A. Motta;E. J. N. Montenegro;T. Stamford;A. Silva;F. R. Silva
研究了暴露于110 mT和220 mT稳态磁场（SMF）的酿酒酵母培养物，以观察最终诱导的生长变化和代谢活性的变化。通过光谱法评价细胞质量（生物量）生长，并根据观察开始和结束时测量的产生的CO2压力和培养基pH值变化估计代谢变化。将在非充气液体琼脂沙氏葡萄糖培养基中培养的酵母菌株DAUFPE-1012暴露于NdFeBr磁体产生的SMF。结果显示，与对照组相比，220 mT SMF诱导的变化为细胞增殖增加（1.84%）和CO2产生增加（36.1%）。此外，220 mT SMF暴露培养物的初始至最终pH值差异高于110 mT SMF和对照值。与其他培养物相比，在220 mT SMF暴露后观察到的整体酸化和CO2产生的增加与生物量生长值不对应，并且显然是由细胞代谢率的增强引起的。该技术在发酵过程中的未来生物技术应用中变得非常有前途。
Saccharomyces cerevisiae cultures exposed to 110 mT and 220 mT steady magnetic fields (SMF) were studied to observe eventual induced growth alterations and changes in metabolic activity. Cell mass (biomass) growth was evaluated by light spectrometry, and metabolic alterations were estimated on the basis of the CO2 pressure produced and by the culture media pH changes, measured at the beginning and the end of the observation. The yeast strain DAUFPE‐1012, cultivated in a nonaerated liquid agar Sabouraud glucose medium, was exposed to SMF generated by NdFeBr magnets. Results showed alterations induced by 220 mT SMF as an increment in cell proliferation (1.84%) and an increased CO2 production (36.1%) as compared to control groups. Furthermore, the initial‐to‐final pH difference in 220 mT SMF exposed cultures was higher than the 110 mT SMF and the control values. The whole acidification and the rise in CO2 production observed after 220 mT SMF exposure did not correspond to the biomass growth values, as compared to the other cultures, and was apparently provoked by a enhancement in the cellular metabolic rate. This technique becomes very promising for future biotechnological applications in fermentative processes.