Distinct adhesive behaviors of neurons and neural precursor cells during regional differentiation in the mammalian forebrain.
Distinct adhesive behaviors of neurons and neural precursor cells during regional differentiation in the mammalian forebrain.
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哺乳动物前脑区域分化过程中神经元和神经前体细胞的独特粘附行为。
DOI：
10.1006/dbio.1995.1140
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
LaMantia,AS
中科院分区：
文献类型：
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作者：
Whitesides3rd,JG;LaMantia,AS
在出现哺乳动物前脑的主要功能分区-新皮质，海马，嗅球，基底神经节和基底前脑-端脑囊泡的侧面的区别是早期神经元分化评估MAP 2和GAP 43的表达和增加的表达的Ca 2 +-非依赖性/免疫球蛋白超家族细胞粘附分子（CAM）NCAM，L1和TAG-1。相反，腹侧和内侧方面的囊泡显示很少的早期神经元分化和中间或不可检测的CAM表达水平。我们问这三个区域的细胞是否获得不同的粘附和识别特性，反映它们的位置，神经元分化状态和CAM表达水平。在解离/再聚集测定中，来自侧端脑囊泡的细胞形成最大的再聚集体，而腹侧再聚集体具有中等大小，并且内侧再聚集体是最小的。这种差异粘附具有Ca 2+非依赖性成分，并且来自每个区域的重新聚集体中的细胞保持CAM和与其起源区域一致的其他神经元标记物的表达。此外，来自端脑外侧的细胞可以特异性地从中间细胞中分离出来。在早期神经元分化和Ca 2 +-独立CAM的表达之前观察到小的粘附性，当前脑仍然是前脑囊泡时，也不遵循可检测CAM表达的模式，一旦前脑雏形形成。因此，在早期发育的前脑细胞获得不同的粘附和识别性能，反映了同时出现的区域差异，神经元分化和CAM表达。这些差异是短暂的，只能检测到端脑囊泡之前和期间的主要前脑分区的雏形的形态发生。
Prior to the emergence of the major functional subdivisions of the mammalian forebrain—the neocortex, hippocampus, olfactory bulb, basal ganglia, and basal forebrain—the lateral aspect of the telencephalic vesicle is distinguished by early neuronal differentiation assessed by MAP2 and GAP43 expression and increased expression of the Ca2+-independent/immunoglobulin superfamily cell adhesion molecules (CAMs) NCAM, L1, and TAG-1. In contrast, the ventral and medial aspects of the vesicle show little early neuronal differentiation and intermediate or undetectable levels of CAM expression. We asked whether cells from these three regions acquire distinct adhesive and recognition properties that reflect their position, state of neuronal differentiation, and level of CAM expression. In a dissociation/reaggregation assay, cells from the lateral telencephalic vesicle form the largest reaggregates while ventral reaggregates are of intermediate size and medial reaggregates are the smallest. This differential adhesion has a Ca2+-independent component, and cells in reaggregates from each region maintain expression of CAMs and other neuronal markers consistent with their region of origin. Furthermore, cells from the lateral telencephalon can specifically sort out from medial cells. Little adhesivity is observed prior to early neuronal differentiation and the expression of Ca2+-independent CAMs, when the forebrain is still a prosencephalic vesicle, nor does it follow the pattern of detectable CAM expression once forebrain rudiments are formed. Thus, cells in the early developing forebrain acquire distinct adhesive and recognition properties that reflect the concurrent emergence of regional differences in neuronal differentiation and CAM expression. These differences are transient and can only be detected in the telencephalic vesicle before and during the morphogenesis of rudiments of major forebrain subdivisions.