Micturition-related neuronal firing in the periaqueductal gray area in cats
Micturition-related neuronal firing in the periaqueductal gray area in cats
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DOI：
10.1016/j.neuroscience.2004.04.033
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发表时间：
2004-01-01
期刊：
影响因子：
3.3
通讯作者：
Hattori, T
中科院分区：
文献类型：
--
作者：
Liu, Z;Sakakibara, R;Hattori, T
中脑导水管周围灰质（PAG）是促进情绪运动反应、生殖行为和镇痛的区域。最近的研究表明，PAG中的神经元可能对调节实验动物和人类的排尿反射至关重要。我们研究了20只去脑猫的PAG和邻近区域的单神经元活动对等容自发排尿反射的反应。在PAG中共记录到84个与储尿/排尿周期相关的神经元。在所记录的神经元中，最常见的是强直性储存神经元（43%），其次是强直性排尿神经元（29%），相位性储存神经元（15%）和相位性排尿神经元（13%）。除了强直/相位以及存储/排尿分类，神经元表现出不同的放电模式：增强，恒定和递减，与恒定放电模式是最常见的。位于PAG内的16个神经元的放电模式与PAG腹侧的神经元相似，排尿神经元分布在更广泛的区域，而存储神经元似乎集中在PAG的中部（P0-1，Horsley-Clarke坐标）。在PAG中施加高频刺激（HFS; 0.2 ms持续时间，100 Hz）引起排尿反射的抑制。用于引起抑制反应的电刺激的有效幅度小于50 μ A。本研究结果表明，PAG的HFS抑制猫的排尿反射，PAG内存在与排尿相关的神经元放电，提示PAG参与排尿的神经控制。(C)2004年IBRO。由爱思唯尔有限公司出版。保留所有权利。
The midbrain periaqueductal gray (PAG) is the area promoting emotional motor responses, reproductive behaviors and analgesia. Recent studies suggest that neurons in the PAG may be crucial for regulating the micturition reflex in both experimental animals and humans. We examined single neuronal activities in the PAG and the adjacent area in response to isovolumetric spontaneous micturition reflexes in 20 supracollicular decerebrated cats. In total, 84 neurons were recorded in the PAG that were related to urinary storage/micturition cycles. Of the neurons recorded, the most common were tonic storage neurons (43%), followed by tonic micturition neurons (29%), phasic storage neurons (15%) and phasic micturition neurons (13%). In addition to the tonic/phasic as well as storage/micturition classification, the neurons showed diverse discharge patterns: augmenting, constant and decrementing, with the constant discharge pattern being most common. Of the 16 neurons located within the PAG that had similar discharge patterns to those just ventral to the PAG, the micturition neurons were distributed in a broader area, whereas the storage neurons seemed to be concentrated in the middle part of the PAG (P0-1, Horsley-Clarke coordinate). High-frequency stimulation (HFS; 0.2-ms duration, 100 Hz) applied in the PAG elicited inhibition of the micturition reflex. Effective amplitude of the electrical stimulation for evoking inhibitory responses was less than 50 muA. In conclusion, the results of the present study showed that HFS of the PAG inhibited the micturition reflex and there were micturition-related neuronal firings in the PAG in cats, suggesting that the PAG is involved in neural control of micturition. (C) 2004 IBRO. Published by Elsevier Ltd. All rights reserved.