Bacillus subtilis B21 and Bacillus licheniformis B26 improve intestinal health and performance of broiler chickens with Clostridium perfringens-induced necrotic enteritis
Bacillus subtilis B21 and Bacillus licheniformis B26 improve intestinal health and performance of broiler chickens with Clostridium perfringens-induced necrotic enteritis
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DOI：
10.1111/jpn.13082
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发表时间：
2019-07-01
影响因子：
2.7
通讯作者：
Yang, Xiaojun
中科院分区：
文献类型：
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作者：
Musa, Bello Bodinga;Duan, Yongle;Yang, Xiaojun
本研究调查了芽孢杆菌益生菌对患有产气荚膜梭菌引起的坏死性肠炎的肉鸡的生产性能和肠道健康的影响。一日龄 Arbor Acre (n = 480) 被随机分配到 4 个处理组，共 10 个笼子，每只 12 只鸡：(a) 基础饮食阴性对照 (NC)，不添加益生菌或抗生素，配方中分别含有 2,930 和 3,060 kcal/kg，CP 为 24.07 和 15.98% CP，分别用于起始日和育肥日粮，(b) 基础日粮 + 恩拉霉素(5 mg/kg)，抗生素生长促进剂(AGP)； (c) 基础饮食 + 枯草芽孢杆菌 B21，每克 2 x 10(9) CFU (BS)； (d) 基础饮食 + 地衣芽孢杆菌 B26，每克 2 x 10(9) CFU (BL)；评估生长性能、肠道形态、肠道病变评分、短链脂肪酸 (SCFA) 和粘膜屏障紧密连接 (TJ) mRNA 表达。 NC 和 BL 饲喂组在第 1 至第 21 日的平均日采食量高于 AGP 和 BS（p = 0.005），而 BS 和 AGP 饲喂组在第 22 至第 42 日以及第 1 至第 42 日龄期间的平均日增重更高。 AGP和NC喂养组的死亡率分别较高（12.5％）和较低（5.5％），BS和BL的病变评分高于AGP，而NC组未观察到病变，结果显示与NC和BS相比，十二指肠和空肠绒毛高度与隐窝深度（VH：CD）较高。益生菌喂养组在攻击后第 21 天 (PC) 表现出比其他组更高的总 (SCFA)、乙酸和丁酸浓度。 BL组十二指肠（d7）PC、空肠（d7）和（d21）PC中claudin-1的表达上调，而在d21 PC时，AGP和BL在空肠和回肠中occlusionns的表达较高。本研究表明，BS和BL在预防或部分预防肉鸡NE效应方面与AGP有一定相似性。
This study investigated the influence of Bacillus-based probiotics on performance and intestinal health in broiler challenged with Clostridium perfringens-induced necrotic enteritis. One-day-old Arbor Acre (n = 480) were randomly assigned to four treatments with 10 cages of 12 birds: (a) basal diet negative control (NC), with no probiotics nor antibiotics formulated to contain 2,930 and 3,060 kcal/kg with 24.07 and 15.98% CP, for starter and finisher diet, respectively, (b) basal diet + enramycin (5 mg/kg), an antibiotic growth promoter (AGP); (c) basal diet + Bacillus subtilis B21 at 2 x 10(9) CFU per g (BS); (d) basal diet + Bacillus licheniformis B26 at 2 x 10(9) CFU per g (BL); growth performance, intestinal morphology, intestinal lesion scores, short-chain fatty acids (SCFAs) and mucosal barrier tight junction's (TJ) mRNA expression were assessed. NC- and BL-fed groups showed higher (p = 0.005) average daily feed intake from d1 to d21 than AGP and BS, whereas BS- and AGP-fed groups showed higher average daily weight gain from d22 to d42 and d1 to d42 of age. Higher mortality rate of (12.5%) and lower of (5.5%) were recorded in AGP and NC fed-groups respectively, lesion score was higher in BS and BL than in AGP, while no lesion was observed in NC group, results revealed higher duodenum and jejunum villus height to crypt depth (VH:CD) compared with NC and BS. Probiotics-fed groups showed higher total (SCFAs), acetic and butyric acid concentrations at d21 post-challenge (PC) than other groups. The expression of claudin-1 was upregulated in duodenum (d7) PC and in jejunum (d7) and (d21) PC in BL group, while at d21 PC, the expression of occludens was higher in jejunum and ileum by AGP and BL. The present study indicated both BS and BL have some similarity with AGP in preventing or partially preventing NE effect in broilers.