The botrydial biosynthetic gene cluster of Botrytis cinerea displays a bipartite genomic structure and is positively regulated by the putative Zn(II)2Cys6 transcription factor BcBot6
The botrydial biosynthetic gene cluster of Botrytis cinerea displays a bipartite genomic structure and is positively regulated by the putative Zn(II)2Cys6 transcription factor BcBot6
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DOI：
10.1016/j.fgb.2016.10.003
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发表时间：
2016-11-01
影响因子：
3
通讯作者：
Viaud, Muriel
中科院分区：
文献类型：
--
作者：
Porquier, Antoine;Morgant, Guillaume;Viaud, Muriel
Botrydial（BOT）是由多食性植物病原真菌灰葡萄孢（Botrytis cinerea）产生的一种非寄主特异性植物毒素。研究了BOT生物合成基因簇的基因组区域，发现了两个额外的基因，名为Bcbot 6和Bcbot 7。分析显示，含有Bcbot基因的G + C/A + T平衡区域与由经历了重复诱导的点突变（RIP）的转座因子的遗迹组成的A + T丰富区域交替。此外，BcBot 6，一个Zn（II）（2）Cys（6）假定的转录因子被鉴定为核蛋白和BOT生物合成的主要正调控因子。此外，Delta Bcbot 6突变体的表型表明，Bcbot 6和因此BOT在B中的发育、致病性和对非生物胁迫的反应中是不稳定的。灰霉菌株B05.10。最后，我们的数据显示，B。假灰蝶也是杂食性的，与B同域生活。cinerea，缺乏产生BOT的能力。BcBot 6作为BOT合成的主要调节因子的鉴定是全面理解BUT合成的完整调节网络及其在B中的生态作用的第一步。灰蝶生活史(C)2016 Elsevier Inc. All rights reserved.
Botrydial (BOT) is a non-host specific phytotoxin produced by the polyphagous phytopathogenic fungus Botrytis cinerea. The genomic region of the BOT biosynthetic gene cluster was investigated and revealed two additional genes named Bcbot6 and Bcbot7. Analysis revealed that the G + C/A + T-equilibrated regions that contain the Bcbot genes alternate with A + T-rich regions made of relics of transposable elements that have undergone repeat-induced point mutations (RIP). Furthermore, BcBot6, a Zn(II)(2)Cys(6) putative transcription factor was identified as a nuclear protein and the major positive regulator of BOT biosynthesis. In addition, the phenotype of the Delta Bcbot6 mutant indicated that BcBot6 and therefore BOT are dispensable for the development, pathogenicity and response to abiotic stresses in the B. cinerea strain B05.10. Finally, our data revealed that B. pseudocinerea, that is also polyphagous and lives in sympatry with B. cinerea, lacks the ability to produce BOT. Identification of BcBot6 as the major regulator of BOT synthesis is the first step towards a comprehensive understanding of the complete regulation network of BUT synthesis and of its ecological role in the B. cinerea life cycle. (C) 2016 Elsevier Inc. All rights reserved.