Early CD69 expression on peripheral blood lymphocytes from children with dengue hemorrhagic fever
Early CD69 expression on peripheral blood lymphocytes from children with dengue hemorrhagic fever
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DOI：
10.1086/315072
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发表时间：
1999-11-01
影响因子：
6.4
通讯作者：
Ennis, FA
中科院分区：
文献类型：
--
作者：
Green, S;Pichyangkul, S;Ennis, FA
最近的报告表明，登革出血热（DHF）的免疫激活细胞因子和可溶性受体检测血液中。本研究的目的是确定哪些细胞类型被激活，可能是负责细胞因子的生产，全血标本从51名泰国儿童出现在72小时内的发热发作和可检测的血浆登革病毒RNA进行了研究，通过流式细胞术。在病程早期，DHF患儿的绝对CD4 T细胞、CD8 T细胞、NK细胞和γ δ T细胞计数均低于登革热（DF）患儿。在发展DHF的儿童中，表达CD69的细胞在CD8 T细胞和NK细胞上的百分比比DE的儿童更多。这些数据直接表明，细胞免疫激活在急性登革热的早期就存在，并且与疾病的严重程度有关。
Recent reports have demonstrated immune activation in dengue hemorrhagic fever (DHF) by cytokine and soluble receptor detection in blood. The goal of this study was to determine which cell types are activated and likely to be responsible for cytokine production, Whole blood specimens from 51 Thai children presenting within 72 h of fever onset and with detectable plasma dengue viral RNA were studied by flow cytometry. Absolute CD4 T cell, CD8 T cell, NK cell, and gamma delta T cell counts were decreased in children with DHF compared with those with dengue fever (DF) early in the course of illness. The percent of cells expressing CD69 was increased on CD8 T cells and NK cells in children who developed DHF more than in those with DE These data directly demonstrate that cellular immune activation is present early in acute dengue and is related to disease severity.