Mitochondria Targeted and Intracellular Biothiol Triggered Hyperpolarized 129Xe Magnetofluorescent Biosensor.
Mitochondria Targeted and Intracellular Biothiol Triggered Hyperpolarized 129Xe Magnetofluorescent Biosensor.
复制标题
线粒体靶向和细胞内生物硫醇触发超极化129Xe磁荧光生物传感器。
DOI：
10.1021/acs.analchem.6b03742
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发表时间：
2017-02
影响因子：
7.4
通讯作者：
Qingbin Zeng;Qianni Guo;Yaping Yuan;Yuqi Yang;Bin Zhang;Lili Ren;Xiaoxiao Zhang;Qing Luo;Maili Liu;L. Bouchard;Xintai Zhou
中科院分区：
文献类型：
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作者：
Qingbin Zeng;Qianni Guo;Yaping Yuan;Yuqi Yang;Bin Zhang;Lili Ren;Xiaoxiao Zhang;Qing Luo;Maili Liu;L. Bouchard;Xintai Zhou
Biothiols such as gluthathione (GSH), cysteine (Cys), homocysteine (Hcy), and thioredoxin (Trx) play vital roles in cellular metabolism. Various diseases are associated with abnormal cellular biothiol levels. Thus, the intracellular detection of biothiol levels could be a useful diagnostic tool. A number of methods have been developed to detect intracellular thiols, but sensitivity and specificity problems have limited their applications. To address these limitations, we have designed a new biosensor based on hyperpolarized xenon magnetic resonance detection, which can be used to detect biothiol levels noninvasively. The biosensor is a multimodal probe that incorporates a cryptophane-A cage as 129Xe NMR reporter, a naphthalimide moiety as fluorescence reporter, a disulfide bond as thiol-specific cleavable group, and a triphenylphosphonium moiety as mitochondria targeting unit. When the biosensor interacts with biothiols, disulfide bond cleavage leads to enhancements in the fluorescence intensity and changes in the 129Xe chemical shift. Using Hyper-CEST (chemical exchange saturation transfer) NMR, our biosensor shows a low detection limit at picomolar (10-10 M) concentration, which makes a promise to detect thiols in cells. The biosensor can detect biothiol effectively in live cells and shows good targeting ability to the mitochondria. This new approach not only offers a practical technique to detect thiols in live cells, but may also present an excellent in vivo test platform for xenon biosensors.
