POLYADENYLATED RNA OF MOUSE OVA AND LOSS OF MATERNAL RNA IN EARLY DEVELOPMENT
POLYADENYLATED RNA OF MOUSE OVA AND LOSS OF MATERNAL RNA IN EARLY DEVELOPMENT
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DOI：
10.1016/0012-1606(80)90048-2
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发表时间：
1980-01-01
影响因子：
2.7
通讯作者：
DELEON, V
中科院分区：
文献类型：
--
作者：
BACHVAROVA, R;DELEON, V
在卵母细胞生长过程中，通过在poly（U）Sepharose柱上的层析，分析含有标记RNA的排卵小鼠卵在poly（A）+和poly（A）- RNA之间的标记分布。对poly（A）- RNA对poly（A）+级分的污染进行了小的校正。在排卵前19-7天卵母细胞生长期的任何时间点，在poly（A）+级分中发现约9.6%的RNA标记。标记的poly（A）RNA在蔗糖梯度上显示约14 S-30 S的异质性谱。根据现有证据计算的小鼠卵中存在的母体mRNA的量是总卵RNA的5-8%，足以显著促进早期胚胎蛋白质合成。标记的母体RNA随后在植入前发育期间在第1天下降了40%，在第2天保持稳定，并在发育至胚泡阶段的第3天再下降了30%。RNA标记物在poly（A）+ RNA中的比例始终保持大致恒定。特定母体信息和核糖体的降解可能伴随着发育第1天蛋白质合成模式的变化。很大一部分母体RNA显然在胚泡形成过程中被使用。
Ovulated mouse ova containing RNA labeled during oocyte growth were analyzed for the distribution of label between poly(A)+ and poly(A)- RNA by chromatography on poly(U) Sepharose columns. A small correction was made for contamination of the poly(A)+ fraction by poly(A)- RNA. About 9.6% of the RNA label was found in the poly(A)+ fraction for RNA labeled at any point throughout the growth phase of the oocyte 19-7 days before ovulation. The labeled poly(A) RNA displayed a heterogeneous profile from about 14 S-30S on sucrose gradients. The amount of maternal mRNA present in the mouse egg calculated from available evidence is 5-8% of the total egg RNA, sufficient to substantially contribute to early embryonic protein synthesis. Labeled maternal RNA followed during preimplantation development declined by 40% on the 1st day, was stable on the 2nd day and declined by an additional 30% on the 3rd day of development to the blastocyst stage. The proportion of RNA label in poly(A)+ RNA remained approximately constant throughout. Degradation of specific maternal messages and ribosomes may accompany changes in the pattern of protein synthesis on the 1st day of development. A substantial proportion of maternal RNA is apparently used during blastocyst formation.