DNA binding of PhoB and its interaction with RNA polymerase
DNA binding of PhoB and its interaction with RNA polymerase
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DOI：
10.1006/jmbi.1996.0298
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发表时间：
1996-05-31
影响因子：
5.6
通讯作者：
Suzuki, M
中科院分区：
文献类型：
--
作者：
Makino, K;Amemura, M;Suzuki, M
我们已经确定了DNA结合域（DBD）的大肠杆菌激活蛋白PhoB的C-末端91个残基。发现PhoB DBD中的四个氨基酸位置对于与含有sigma（70）亚基的RNA聚合酶全酶的相互作用是重要的。假设PhoB DBD在结构上类似于组蛋白H5 DBD，四个位置被放置在连接两个推定螺旋2和3的转弯区域周围（螺旋3可能是识别螺旋）。在pstS启动子中鉴定了PhoB的结合位点，其中三个具有TGTCA序列，一个具有TTACA序列。pstS启动子具有内在弯曲（或可弯曲性），其在结合PhoB时大大增强。在此基础上，对PhoB-DNA-RNA聚合酶相互作用的一些方面进行了讨论。(C)出版社：Academic Press Limited。
We have identified the DNA-binding domain (DBD) of an Escherichia coli activator protein PhoB as its C-terminal 91 residues. Four amino acid positions in the PhoB DBD are found important for interaction with the RNA polymerase holoenzyme that contains the sigma(70) subunit. Assuming that the PhoB DBD is structurally similar to the histone H5 DBD, the four positions are placed around the turn region that connects two putative helices, 2 and 3 (helix 3 is likely to be the recognition helix). The binding sites of PhoB, three with the sequence TGTCA and one of TTACA, are identified in the pstS promoter. The pstS promoter has intrinsic bending (or bendability), which is much enhanced upon binding PhoB. On the basis of the above, some aspects of the PhoB-DNA-RNA polymerase interaction are discussed. (C) 1996 Academic Press Limited.