Effects of quercetin on gene and protein expression of NOX and NOS after myocardial ischemia and reperfusion in rabbit.
Effects of quercetin on gene and protein expression of NOX and NOS after myocardial ischemia and reperfusion in rabbit.
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DOI：
10.1111/j.1755-5922.2009.00071.x
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发表时间：
2009-03
影响因子：
3.1
通讯作者：
L. Wan;J. Xia;Duoyun Ye;Jinping Liu;Jun Chen;G. Wang
中科院分区：
文献类型：
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作者：
L. Wan;J. Xia;Duoyun Ye;Jinping Liu;Jun Chen;G. Wang
先前的研究表明，活性氧（ROS）、内皮型一氧化氮合酶（eNOS）和诱导型一氧化氮合酶（iNOS）参与心肌缺血再灌注损伤（MIRI）的病理生理学。 NADPH 氧化酶的 NOX 家族具有产生超氧化物和 ROS 的能力。多项研究表明，槲皮素对 MIRI 具有保护作用。我们的目的是研究槲皮素对兔子 MIRI 后 NOX2、eNOS 和 iNOS 的影响。新西兰兔经历 30 分钟的心肌缺血，然后再灌注 12 小时。然后他们被随机分配到四个实验组：对照组、I/R（缺血/再灌注）、槲皮素（Que）、I/R + Que。比较 NOX2、eNOS 和 iNOS 的基因和蛋白表达。在实时 PCR 和 Western blotting 研究中，心肌缺血再灌注诱导的 NOX2 和 iNOS 表达均增强（P < 0.01），但 I/R 心脏中 eNOS mRNA 和蛋白表达与对照组无显着差异（P < 0.01）。给予槲皮素可降低对照心脏和 I/R 心脏中的 NOX2、eNOS 和 iNOS mRNA 和蛋白表达（P < 0.01）。 MIRI 后 NOX2 和 iNOS 的基因和蛋白表达增加。槲皮素不仅抑制心肌缺血再灌注诱导的NOX2和iNOS mRNA和蛋白表达，而且还抑制eNOS mRNA和蛋白表达。
Previous studies have suggested that reactive oxygen species (ROS), endothelial nitric oxide synthase (eNOS), and inducible nitric oxide synthase (iNOS) are involved in the pathophysiology of myocardial ischemia-reperfusion injury (MIRI). The NOX family of NADPH oxidases share the capacity to generate superoxide and ROS. Several studies have demonstrated that quercetin possesses a protective effect against MIRI. Our aim is to investigate the effects of quercetin on NOX2, eNOS, and iNOS after MIRI in rabbits. New Zealand rabbits were subjected to 30 min of myocardial ischemia followed by 12 h of reperfusion. They were then randomly assigned to four experimental groups: control, I/R (ischemia/reperfusion), quercetin (Que), I/R + Que. Gene and protein expression of NOX2, eNOS, and iNOS were compared. Both in real-time PCR and in the Western blotting studies, myocardial ischemia-reperfusion-induced NOX2 and iNOS expression were enhanced (P < 0.01) but eNOS mRNA and protein expression in I/R hearts were not significantly different from those in control (P < 0.01). Administration of quercetin reduced NOX2, eNOS, and iNOS mRNA and protein expression both in control and in I/R heart (P < 0.01). Gene and protein expression of NOX2 and iNOS were increased after MIRI. Quercetin not only inhibited myocardial ischemia-reperfusion-induced NOX2 and iNOS mRNA and protein expression but also inhibited eNOS mRNA and protein expression.