Apoptosis and porcine reproductive and respiratory syndrome virus
Apoptosis and porcine reproductive and respiratory syndrome virus
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DOI：
10.1016/j.vetimm.2004.09.004
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发表时间：
2004-12-08
影响因子：
1.8
通讯作者：
Fox, JM
中科院分区：
文献类型：
--
作者：
Miller, LC;Fox, JM
尽管大量研究探讨了猪繁殖与呼吸综合征病毒（PRRSV）感染细胞可能诱导细胞凋亡，但PRRSV感染是否直接诱导细胞凋亡尚不清楚。有明确的证据表明，prrsv感染猪的组织中存在凋亡细胞。然而，许多研究未能证明凋亡细胞被PRRSV感染。这导致一些研究人员提出，在PRRSV感染期间，“旁观者”细胞，而不是被感染的细胞，以一种尚未确定的机制发生凋亡。为了确定PRRSV复制是否触发细胞凋亡反应，还需要研究PRRSV感染诱导凋亡基因表达的反应。我们利用微阵列和半定量反转录聚合酶链反应（sqRT-PCR）来评估prrsv感染的MARC-145细胞中凋亡基因的表达。在感染的前24小时内检测了26个凋亡相关基因，发现它们没有改变，这表明prrsv感染的细胞没有发生凋亡诱导。此外，通过检测游离核体复合物，我们检测了不同感染倍数下PRRSV感染导致的细胞凋亡和坏死死亡。本研究表明，prrsv感染的MARC-145细胞坏死水平远高于凋亡水平，并且随着感染细胞的病毒水平的增加而增加。Elsevier B.V.出版
Despite numerous studies examining the possible induction of apoptosis in porcine reproductive and respiratory syndrome virus (PRRSV)-infected cells, it remains unclear if PRRSV infection results in direct apoptotic induction. There is clear evidence that apoptotic cells are present in tissues from PRRSV-infected pigs. However, many of these studies have failed to show that the apoptotic cells are infected with PRRSV. This has led some investigators to propose that "bystander" cells, not infected cells, become apoptotic during PRRSV infection by a yet undetermined mechanism. Studies examining the induction of the apoptotic gene expression response to PRRSV infection are needed to determine if PRRSV replication triggers an apoptotic response. We have utilized microarray and semi -quantitative reverse-transcription polymerase chain reaction (sqRT-PCR) to evaluate apoptotic gene expression in PRRSV-infected MARC-145 cells. Twenty-six apoptosis-related genes were examined during the first 24 h of infection and found to be unaltered, indicating that apoptotic induction was not occurring in PRRSV-infected cells. Additionally, using detection of free nucleosomal complexes, we examined cells for both apoptotic and necrotic death resulting from PRRSV infection at varying multiplicities of infection. This study indicates that PRRSV-infected MARC-145 cells undergo necrosis at a much higher level than apoptosis, and increases with virus levels used to infect the cells. Published by Elsevier B.V.