Edwardsiella tarda outer membrane protein 1 is a virulence factor and a protective immunogen
Edwardsiella tarda outer membrane protein 1 is a virulence factor and a protective immunogen
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DOI：
10.1016/j.aquaculture.2021.736596
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发表时间：
2021-03
期刊：
影响因子：
4.5
通讯作者：
Cheng Jin;Mo-fei Li
中科院分区：
文献类型：
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作者：
Cheng Jin;Mo-fei Li
细菌外膜蛋白是细菌细胞的重要组成部分。迟缓爱德华氏菌（Edwardsiellatarda）是一种重要的水产养殖动物病原菌。在这项研究中，我们研究了外膜蛋白的功能。tarda（命名为Omp1Et）。Omp 1Et定位于E. tarda，重组Omp1Et（rOmp1Et）能够与比目鱼外周血白细胞相互作用。与野生型相比，敲除omp1Et的TX01Δ omp1在运动性、生物膜形成、对宿主细胞的粘附和侵袭、在宿主组织中的播散以及诱导宿主死亡等方面均显著降低。此外，TX01Δ omp1在低pH和氧化条件下的存活能力明显受损。将rOmp1Et作为亚单位疫苗导入比目鱼体内，诱导了较强的抗E的免疫保护。延迟激发。这些结果表明Omp1E是大肠杆菌的表面毒力因子。tarda和rOmp1Et可作为一种有效免疫保护疫苗。
The bacterial outer membrane proteins are important components of bacterial cell. Edwardsiella tarda is a vital bacterial pathogen to a wide range of aquaculture animals. In this study, we examined the function of an outer membrane protein ofE. tarda(named Omp1Et). Omp1Etwas found to localize on the surface ofE. tarda, and recombinant Omp1Et(rOmp1Et) was able to interact with flounder peripheral blood leukocytes. Compared to the wild type, theomp1Etknockout TX01Δomp1was significantly reduced in motility, biofilm formation, attachment and invasion into host cells, dissemination in host tissues, and inducing host mortality. Furthermore, TX01Δomp1was significantly impaired in the ability to survive under low pH and oxidizing conditions. When introduced into flounder as a subunit vaccine, rOmp1Etinduced strong immune protection againstE. tardachallenge. Taken together, these results indicate that Omp1Etis a surface-located virulence factor ofE. tardaand rOmp1Etcan induce effective immune-protection as a submit vaccine.