Analysis of the expression of mir-34a, mir-199a, mir-30c and mir-19a in peripheral blood CD4+T lymphocytes of relapsing-remitting multiple sclerosis patients
Analysis of the expression of mir-34a, mir-199a, mir-30c and mir-19a in peripheral blood CD4+T lymphocytes of relapsing-remitting multiple sclerosis patients
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DOI：
10.1016/j.gene.2018.03.035
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发表时间：
2018-06-15
期刊：
影响因子：
3.5
通讯作者：
Nasr-Esfahani, Mohammad Hossein
中科院分区：
文献类型：
--
作者：
Ghadiri, Nooshin;Emamnia, Negaralsadat;Nasr-Esfahani, Mohammad Hossein
背景：多发性硬化症是一种免疫介导的中枢神经系统炎症性疾病。microrna在ms等自身免疫性疾病中发挥重要作用目的：目的是评估ms复发期和缓解期外周血源性CD4 + T淋巴细胞中miR-34a、miR-199a、miR-30c和miR-19a的表达模式。方法：采集40例RRMS患者（20例复发期和20例缓解期）和20名健康志愿者的血液样本。分离CD4 + T细胞。检测miR-34a、miR-199a、miR-30c、miR-19a的表达水平及Th17、Treg细胞的百分比。我们还对Th17和Treg细胞的主转录因子以及这些mirna的几个靶点的表达进行了评估。结果：数据显示miR-34a、miR-30c和miR-19a在复发期表达升高，miR-199a在缓解期表达降低。ROC曲线数据通过定义复发和缓解期，以及在提出的最佳截止点具有高特异性和敏感性的健康病例，增加了miR-34a、miR-199a、miR-30c和miR-19a的其他重要信息。结论：总的来说，我们发现了MS不同阶段的4种mirna与Th17细胞的分化途径之间存在相关性，它们是MS中最重要的参与者。
Background: Multiple sclerosis is an immune-mediated inflammatory disease of central nervous system. MicroRNAs play important roles in autoimmune diseases such as MS. Objectives: The aim was to evaluate the expression pattern of miR-34a, miR-199a, miR-30c and miR-19a in peripheral blood derived CD4 + T lymphocytes of both relapsing and remitting phases of MS.Methods: Blood samples from 40 RRMS patients (20 in relapsing and 20 in remitting phase) and 20 healthy volunteers were taken. CD4 + T cells were isolated. The expression level of miR-34a, miR-199a, miR-30c and miR-19a, and the percentage of Th17 and Treg cells were measured. Expression of master transcription factors of Th17 and Treg cells and several targets of these miRNAs were also evaluated.Results: Data indicated an increased expression of miR-34a, miR-30c and miR-19a in relapsing phase and decreased expression of miR-199a in remitting phase. ROC curve data add other prestigious information of miR-34a, miR-199a, miR-30c and miR-19a by defining relapsing and remitting phase and also healthy cases with high specificity and sensitivity at a proposed optimum cut-off point.Conclusion: Collectively, we showed a correlation between the four miRNAs with different phases of MS and their possible involvement in differentiation pathways of Th17 cells, as the most important players in MS.