Brain-wide analysis of the supraspinal connectome reveals anatomical correlates to functional recovery after spinal injury.
Brain-wide analysis of the supraspinal connectome reveals anatomical correlates to functional recovery after spinal injury.
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DOI：
10.7554/elife.76254
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发表时间：
2022-07-15
期刊：
影响因子：
7.7
通讯作者：
Blackmore, Murray G.
中科院分区：
文献类型：
--
作者：
Wang, Zimei;Romanski, Adam;Mehra, Vatsal;Wang, Yunfang;Brannigan, Matthew;Campbell, Benjamin C.;Petsko, Gregory A.;Tsoulfas, Pantelis;Blackmore, Murray G.
关键词：
棘上连接体对正常行为和体内平衡至关重要，它由许多从大脑到脊髓的感觉、运动和自主神经投射组成。棘上控制及其损伤后恢复的研究主要集中在少数几个携带运动命令的主要种群上，而对提供自主或关键运动调节的数十个种群的考虑有限。在这里，我们组装了一个实验工作流程来快速描述成年小鼠的整个椎上中叶连接组，并在一个基于网络的资源中传播输出。优化的病毒标记、3D成像和小鼠数字神经解剖图谱的注册将数万个棘上神经元分配到69个已确定的区域。我们证明了这种方法能够澄清脊柱水平之间地形映射的要点，测量人群对脊柱损伤的特异性敏感性，并测试区域特异性神经元保留与功能恢复变异性之间的关系。这项工作将通过扩大对重要但未充分研究的椎骨上种群的理解来促进进展。
The supraspinal connectome is essential for normal behavior and homeostasis and consists of numerous sensory, motor, and autonomic projections from brain to spinal cord. Study of supraspinal control and its restoration after damage has focused mostly on a handful of major populations that carry motor commands, with only limited consideration of dozens more that provide autonomic or crucial motor modulation. Here, we assemble an experimental workflow to rapidly profile the entire supraspinal mesoconnectome in adult mice and disseminate the output in a web-based resource. Optimized viral labeling, 3D imaging, and registration to a mouse digital neuroanatomical atlas assigned tens of thousands of supraspinal neurons to 69 identified regions. We demonstrate the ability of this approach to clarify essential points of topographic mapping between spinal levels, measure population-specific sensitivity to spinal injury, and test the relationships between region-specific neuronal sparing and variability in functional recovery. This work will spur progress by broadening understanding of essential but understudied supraspinal populations.