Metabolite profiling of ginsenosides in rat plasma, urine and feces by LC-MS/MS and its application to a pharmacokinetic study after oral administration of Panax ginseng extract
Metabolite profiling of ginsenosides in rat plasma, urine and feces by LC-MS/MS and its application to a pharmacokinetic study after oral administration of Panax ginseng extract
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大鼠血浆、尿液和粪便中人参皂苷的 LC-MS/MS 代谢分析及其在口服人参提取物后药代动力学研究中的应用
DOI：
10.1002/bmc.4105
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发表时间：
2018-03-01
影响因子：
1.8
通讯作者：
Jiang, Jun
中科院分区：
文献类型：
--
作者：
Dong, Wei-Wei;Han, Xiong-Zhe;Jiang, Jun
人参是一种功能性食品，以茶、粉、胶囊等形式被广泛食用，并具有一系列药理活性，包括适应性、免疫调节、抗肿瘤、抗衰老和抗炎作用。本研究的目的是使用LC-MS/MS鉴定和定量大鼠血浆，尿液和粪便中的人参皂苷及其代谢产物。我们收集了大鼠血浆样品在0.5，1，2，4，8，12，24和48小时，尿液和粪便样品在24小时内积累的量。在粪便样本中观察到14种主要的皂苷及其代谢产物，但在尿液样本中检测到11种低水平的皂苷。研究了主要皂苷及其代谢产物在血浆中的药代动力学。结果表明，化合物K的血药峰浓度、达峰时间和曲线下面积均显著大于其他皂苷。本研究为人参的药物开发和临床应用提供了有价值的信息。
Panax ginseng is widely consumed as a functional food in the form of tea, powder, capsules, among others, and possesses a range of pharmacological activities including adaptogenic, immune-modulatory, anti-tumor, anti-aging and anti-inflammatory effects. The aim of this study was to identify and quantify the major ginsenosides and their metabolites in rat plasma, urine and feces after administration of P. ginseng extract using LC-MS/MS. We collected rat plasma samples at 0.5, 1, 2, 4, 8, 12, 24 and 48h, and the amounts of urine and fecal samples accumulated in 24h. Fourteen major ginsenosides and their metabolites were observed in fecal samples at high levels; however, low levels of 11 ginsenosides were detected in urine samples. The pharmacokinetics of the major ginsenosides and their metabolites was investigated in plasma. The results indicated that the maximum plasma concentration, time to maximum concentration and area under the curve of compound K were significantly greater than those of other ginsenosides. This study thus provides valuable information for drug development and clinical application of P. ginseng.