MbIDGF, a novel member of the imaginal disc growth factor family in Mamestra brassicae, stimulates cell proliferation in two lepidopteran cell lines without insulin.
MbIDGF, a novel member of the imaginal disc growth factor family in Mamestra brassicae, stimulates cell proliferation in two lepidopteran cell lines without insulin.
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DOI：
10.1016/j.ibmb.2006.04.002
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发表时间：
2006-07
影响因子：
3.8
通讯作者：
Jun Zhang;Sachio Iwai;T. Tsugehara;M. Takeda
中科院分区：
文献类型：
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作者：
Jun Zhang;Sachio Iwai;T. Tsugehara;M. Takeda
成虫盘生长因子（IDGF）是一种可溶性多肽生长因子，首先从果蝇成虫盘C1.8+细胞的条件培养基中鉴定。IDGF与胰岛素协同作用，刺激培养的成虫盘细胞生长，提示IDGF可能是果蝇胰岛素或胰岛素样肽的辅因子。在这里，我们报告了IDGF家族的一个新成员，命名为MbIDGF，从甘蓝夜蛾，Mamestra brassicae。利用克隆的MbIDGF cDNA，在杆状病毒感染的Sf 9细胞中表达重组MbIDGF蛋白并纯化。在没有胰岛素的情况下，重组MbIDGF蛋白刺激来自M. brassicae，以剂量依赖的方式。MbIDGF分别在80 ng/ml（0.8nM）和300 ng/ml（6 nM）时对两种细胞的生长刺激达到饱和。这些结果表明，MbIDGF可能通过一种新的机制促进鳞翅目昆虫细胞的生长，而不与胰岛素途径相关。
Imaginal disc growth factor (IDGF) is a soluble polypeptide growth factor that was first identified from the conditioned medium of Drosophilia imaginal disc C1.8+ cells. Working with insulin, IDGF stimulated the growth of cultured imaginal disk cells, which suggested that IDGF might function as a cofactor of Drosophila insulin or insulin like peptide. Here we report a new member of the IDGF family, named MbIDGF, from the cabbage armyworm, Mamestra brassicae. Using a cloned cDNA of MbIDGF, recombinant MbIDGF protein was expressed in baculovirus-infected Sf9 cells and purified. Without insulin, the recombinant MbIDGF protein stimulated cell growth of SES-MaBr-4 and NIAS-MaBr-93 cell lines that were derived from the fat bodies and hemocytes of M. brassicae, in a dose-dependent manner. The saturation of growth stimulation by MbIDGF was attained for the two types of cells at 80ng/ml (0.8nM) and 300ng/ml (6nM), respectively. The results suggest that MbIDGF may stimulate the growth of lepidopteran cells by a new mechanism without associating with the insulin pathway.