Growth stimulative effect of parathyroid hormone, calcitonin and N6,O2'-dibutyryl adenosine 3';5'-cyclic monophosphoric acid on chick embryonic cartilage cultivated in a chemically defined medium.
Growth stimulative effect of parathyroid hormone, calcitonin and N6,O2'-dibutyryl adenosine 3';5'-cyclic monophosphoric acid on chick embryonic cartilage cultivated in a chemically defined medium.
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甲状旁腺激素、降钙素和 N6,O2-二丁酰腺苷 3;5-环单磷酸对化学成分确定的培养基中培养的鸡胚胎软骨的生长刺激作用。
DOI：
10.1507/endocrj1954.27.349
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Hiroyoshi Endo
中科院分区：
文献类型：
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作者：
Kohtaro Kawashima;Shinjiro Iwata;Hiroyoshi Endo
PTH（0.5单位/ml）和CT（0.5单位/ml）强烈刺激鸡胚股骨的体外生长，表现为伸长和干重增加，以及股骨蛋白质、己糖胺、羟脯氨酸、DNA和RNA含量的增加。当PTH和CT同时添加到培养基中时观察到协同效应，这表明其中一种激素的生长刺激作用的机制可能与另一种不同。 PTH暴露于股骨导致环AMP含量增加。直接向培养基中添加二丁酰环 AMP (1 mM) 模拟 PTH 对软骨的作用。因此，PTH 似乎可以通过增加股骨环磷酸腺苷含量来刺激软骨生长。 CT对股骨环AMP含量没有影响。
PTH (0.5 unit/ml) and CT (0.5 unit/ml) strongly stimulated the in vitro growth of the chick embryo femur in terms of elongation and dry weight increase as well as an increase in the protein, hexosamine, hydroxyproline, DNA and RNA content of the femur. A synergistic effect was observed when PTH and CT were simultaneously added to the medium, suggesting that the mechanism of the growth stimulating effect of one of these hormones might be different from that of the other. The exposure of PTH to the femur caused an increase in cyclic AMP content. The direct addition of dibutyryl cyclic AMP (1 mM) to the medium mimicked the effect of PTH on the cartilage. PTH, therefore, seems to stimulate the growth of cartilage via the increase in cyclic AMP content of the femur. CT had no effect on the cyclic AMP content of the femur.