Realizing the Principles for Remote and Selective Detection of Cancer Cells Using Magnetic Nanowires
Realizing the Principles for Remote and Selective Detection of Cancer Cells Using Magnetic Nanowires
复制标题
实现利用磁性纳米线远程选择性检测癌细胞的原理
DOI：
10.1021/acs.jpcb.1c04394
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Stadler, Bethanie
中科院分区：
文献类型：
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作者：
Zamani Kouhpanji, Mohammad Reza;Nemati, Zohreh;Modiano, Jaime;Franklin, Rhonda;Stadler, Bethanie
对生物实体的选择性和远程检测的未满足需求促使纳米生物技术优先考虑可远程检测的生物标签的创新。磁性纳米线（MNWs）被认为是有前途的远程检测，因为磁场可以深入和安全地穿透组织。然而，磁特征的重叠性质一直是选择性检测的长期挑战，我们在这里解决了这个问题。为此，我们合成了13种具有独特不可逆开关场（ISF）特征的MNWs，用于标记犬骨肉瘤（OSCA-8）癌细胞（1组）和聚碳酸酯生物聚合物（12组）。在表征了每种MNW类型的ISF特征后，将MNW标记的癌细胞转移到MNW标记的生物聚合物上，以确定最可区分的ISF特征，并辨别出可靠的选择性检测生物实体的原则。我们表明，通过调整MNWs的矫顽力来调整其ISF是一种非常有效的方法，可以产生不同的磁性生物标记，用于选择性检测细胞。这些发现为纳米生物技术的发展铺平了道路，使使用MNWs进行生物实体的远程和选择性检测成为可能。
The unmet demand for selective and remote detection of biological entities has urged nanobiotechnology to prioritize the innovation of biolabels that can be remotely detected. Magnetic nanowires (MNWs) have been deemed promising for remote detection as the magnetic fields can deeply and safely penetrate into tissue. However, the overlapping nature of the magnetic signatures has been a long-standing challenge for selective detection, which we resolve here. To do so, 13 types of MNWs with unique irreversible switching field (ISF) signatures were synthesized for labeling canine osteosarcoma (OSCA-8) cancer cells (one set) and polycarbonate biopolymers (12 sets). After characterizing the ISF signature of each MNW type, the MNW-labeled cancer cells were transferred onto MNW-labeled biopolymers to determine the most distinguishable ISF signatures and to discern the principles for reliable selective detection of biological entities. We show that tailoring the ISF of MNWs by tuning their coercivity is a highly effective approach for generating distinct magnetic biolabels for selective detection of cells. These findings smooth the path for the progression of nanobiotechnology by enabling the remote and selective detection of biological entities using MNWs.