Unidirectional Cre-mediated genetic inversion in mice using the mutant loxP pair lox66/lox71 -: art. no. e140
Unidirectional Cre-mediated genetic inversion in mice using the mutant loxP pair lox66/lox71 -: art. no. e140
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DOI：
10.1093/nar/gng140
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发表时间：
2003-11-15
影响因子：
14.9
通讯作者：
Rajewsky, K
中科院分区：
文献类型：
--
作者：
Oberdoerffer, P;Otipoby, KL;Rajewsky, K
Cre/loxP重组系统是通过缺失或倒位loxP侧翼DNA片段以条件方式改变小鼠基因组的常用工具。虽然Cre介导的缺失基本上是单向的，但倒位是可逆的，因此不允许组成型表达Cre的细胞中基因功能的稳定改变。定点突变产生了一对不对称的loxP位点（lox 66和lox 71），显示出有利的正向反应平衡。在这里，我们证明，lox 66/lox 71介导的有效和主要是单向反转的开关基板靶向小鼠基因组结合诱导型或细胞类型特异性的cre转基因在体内。
The Cre/loxP recombination system is a commonly used tool to alter the mouse genome in a conditional manner by deletion or inversion of loxP-flanked DNA segments. While Cre-mediated deletion is essentially unidirectional, inversion is reversible and therefore does not allow the stable alteration of gene function in cells that constitutively express Cre. Site-directed mutagenesis yielded a pair of asymmetric loxP sites (lox66 and lox71) that display a favorable forward reaction equilibrium. Here, we demonstrate that lox66/lox71 mediates efficient and predominantly unidirectional inversion of a switch substrate targeted to the mouse genome in combination with either inducible or cell type-specific cre-transgenes in vivo.