Onchocerca volvulus:: Limited heterogeneity in the nuclear and mitochondrial genomes
Onchocerca volvulus:: Limited heterogeneity in the nuclear and mitochondrial genomes
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DOI：
10.1006/expr.1999.4450
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发表时间：
1999-12-01
影响因子：
2.1
通讯作者：
Unnasch, TR
中科院分区：
文献类型：
--
作者：
Keddie, EM;Higazi, T;Unnasch, TR
西非热带雨林和热带稀树草原生物气候特有的盘尾丝虫（Onchocerca volvulus）西非种群在感染个体中诱导眼部疾病的能力不同。近年来，基于临床和动物模型的研究都表明，特定的寄生虫抗原与眼盘尾丝虫病的发展有关。为了验证这一假设，即致盲和非致盲寄生虫的致病潜力的差异可能反映在定性差异的抗原，已牵连到眼部盘尾丝虫病的发展，我们比较了序列的两个寄生虫抗原牵连眼病致盲和非致盲株寄生虫。结果表明，这些基因的寄生虫菌株之间的高度同质性。这项研究扩展到包括额外的核基因编码抗原，通常被感染O。肠扭转和寄生虫的线粒体基因组。结果表明，O.从非洲和美洲的几个不同地点收集的肠扭转分离株。这种高度的遗传同质性可能反映了寄生虫通过最近的遗传瓶颈。(C)北京：科学出版社.
West African populations of Onchocerca volvulus endemic to the rain forest and savanna bioclimes of West Africa differ in their ability to induce ocular disease in infected individuals. in recent years, both clinical- and animal-model-based studies have implicated particular parasite antigens in the development of ocular onchocerciasis. To test the hypothesis that the difference in pathogenic potential of blinding and nonblinding parasites might be reflected in qualitative differences in antigens that have been implicated in the development of ocular onchocerciasis, we compared the sequences of two parasite antigens implicated in the development of ocular disease in blinding- and nonblinding-strain parasites. The results demonstrated a high level of homogeneity between the parasite strains in these genes. The study was extended to include additional nuclear genes encoding antigens that are commonly recognized by individuals infected with O. volvulus and to the mitochondrial genome of the parasite. The results demonstrate a high degree of homogeneity in both the nuclear and the mitochondrial genomes among O. volvulus isolates collected from several different sites in Africa and in the Americas. This high degree of genetic homogeneity may reflect the passage of the parasite through a recent genetic bottleneck. (C) 1999 Academic Press.