Differences in structure and dynamics of Polynucleobacter communities in a temperate and a subtropical lake, revealed at three phylogenetic levels
Differences in structure and dynamics of Polynucleobacter communities in a temperate and a subtropical lake, revealed at three phylogenetic levels
复制标题
DOI：
10.1111/j.1574-6941.2006.00105.x
复制
发表时间：
2006-07-01
影响因子：
4.2
通讯作者：
Hahn, Martin W.
中科院分区：
文献类型：
--
作者：
Wu, Qinglong L.;Hahn, Martin W.
通过非培养方法调查细菌的生态学研究结果预计将受到所应用的分子工具的系统发育分辨率的影响。这种潜在的影响是在东亚亚热带大型、浅水、部分富营养化的太湖和温带中欧的蒙得湖的上层多核杆菌集群细菌(BetaProtebacteria)的比较群落研究中得到的。这两个不同的栖息地是在12个月的同一时期内进行采样的。通过与一组针对Beta II分支(包括多核细菌簇)、类属单系多核细菌簇和4个类种亚簇的嵌套探针进行荧光原位杂交，在三个系统发育水平上研究了群落的动态。在调查期间，B亚类群是两个湖泊中数量最多的亚类群，但在两个生境中表现出不同的种群动态。对研究中的两个湖泊和之前调查的一个酸性池塘中的多核细菌群落结构的生境间比较表明，在系统发育狭窄的多核细菌集群中存在生态多样性。这些结果可以通过亚星系团特有的探针获得，但较低系统发育分辨率的探针可能会错过这些结果。
The results of ecological studies investigating bacteria by cultivation-independent methods are expected to be influenced by the phylogenetic resolution of the applied molecular tools. This potential influence was investigated in a comparative community study on Polynucleobacter cluster bacteria (Betaproteobacteria) inhabiting the pelagic of the large, shallow, partially hypertrophic Taihu Lake located in subtropical East Asia, and the deep oligo-mesotrophic Lake Mondsee located in temperate Central Europe. The two contrasting habitats were sampled over the same period of 12 months. The community dynamics were investigated at three phylogenetic levels by fluorescent in situ hybridizations with a set of nested probes specific for the beta II clade (including the Polynucleobacter cluster), the genus-like monophyletic Polynucleobacter cluster, and four species-like subclusters. Subcluster B was the numerically dominating subcluster in both lakes over the investigation periods, but demonstrated different population dynamics in the two habitats. Interhabitat comparisons of the Polynucleobacter community structure in the two lakes in the study, and a previously investigated acidic pond indicated ecological diversification within the phylogenetically narrow Polynucleobacter cluster. These results could be obtained by help of the subcluster-specific probes, but would have been missed with probes of a lower phylogenetic resolution.