Ex Vivo Expansion of Murine and Human Hematopoietic Stem Cells
Ex Vivo Expansion of Murine and Human Hematopoietic Stem Cells
复制标题
DOI：
10.1007/978-1-4939-1133-2_14
复制
发表时间：
2014-01-01
期刊：
影响因子：
--
通讯作者：
Chute, John P.
中科院分区：
文献类型：
--
作者：
Doan, Phuong L.;Chute, John P.
造血干细胞具有自我更新的能力，并在生物体的整个生命周期中产生完整的成熟血液和免疫系统。在这里，我们描述了分离和培养小鼠骨髓（BM）CD 34（-）ckit（+）Sca 1（+）谱系-（CD 34-KSL）造血干细胞（HSC）的方法。我们还描述了一种通过竞争性再增殖试验来测量功能性HSC含量的方法。此外，我们总结了分离和培养人CD 34（+）CD 38（-）谱系（-）细胞的方法，这些细胞富集了人造血干细胞和祖细胞。
Hematopoietic stem cells have the capacity to self-renew and give rise to the entirety of the mature blood and immune system throughout the lifespan of an organism. Here, we describe methods to isolate and culture murine bone marrow (BM) CD34(-)ckit(+) Sca1(+) Lineage - (CD34 -KSL) hematopoietic stem cells (HSCs). We also describe a method to measure functional HSC content via the competitive repopulation assay. Furthermore, we summarize methods to isolate and culture human CD34(+) CD38(-)Lineage(-)cells which are enriched for human hematopoietic stem and progenitor cells.