Non-biofouling materials prepared by atom transfer radical polymerization grafting of 2-methacryloloxyethyl phosphorylcholine: Separate effects of graft density and chain length on protein repulsion
Non-biofouling materials prepared by atom transfer radical polymerization grafting of 2-methacryloloxyethyl phosphorylcholine: Separate effects of graft density and chain length on protein repulsion
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DOI：
10.1016/j.biomaterials.2005.07.006
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发表时间：
2006-02-01
期刊：
影响因子：
14
通讯作者：
Zhu, SP
中科院分区：
文献类型：
--
作者：
Feng, W;Brash, JL;Zhu, SP
采用引发剂自组装和表面引发原子转移自由基聚合(ATRP)相结合的方法，从硅片表面制备了接枝密度为0.06~0.39链/nm(2)、链长为5~200个单体单元的仿生聚(2-甲基丙烯酰氧乙基磷酰胆碱)刷子。利用水接触角、X射线光电子能谱和原子力显微镜对改性后的表面进行了表征。测试了这些具有良好控制的聚(MPC)刷子的表面的蛋白质排斥性能。在pH 7.4时，纤维蛋白原在三缓冲液中的吸附随聚(MPC)接枝密度和/或链长的增加而显著降低，在接枝密度和链长分别为0.29链/nm(2)和100个单位时达到约10 ng/cm(2)，而未改性的样品约为570 ng/cm(2)。虽然纤维蛋白原的吸附同时由接枝密度和链长决定，但它对接枝密度的依赖性强于对链长的依赖。(C)2005爱思唯尔有限公司。保留所有权利。
Biomimetic poly(2-methacryloyloxyethyl phosphorylcholine) (poly(MPC)) brushes with graft density 0.06-0.39 chains/nm(2) and chain length 5-200 monomer units were prepared from silicon wafer surfaces by combining self-assembly of initiator and surface-initiated atom transfer radical polymerization (ATRP). Water contact angle, X-ray photoelectron spectroscopy, and atomic force microscopy were used to characterize the modified surfaces. These surfaces with well-controlled poly(MPC) brushes were tested for protein repelling performance. Fibrinogen adsorption from tris-buffered saline at pH 7.4 decreased significantly with increasing graft density and/or chain length of poly(MPC) and reached a level of < 10 ng/cm(2) at graft density >= 0.29 chains/nm(2) and chain length >= 100 units, compared to ca. 570 ng/cm(2) for the unmodified samples. While the fibrinogen adsorption was determined by both graft density and chain length, it showed a stronger dependence on graft density than on chain length. (c) 2005 Elsevier Ltd. All rights reserved.