Microscopic observations on the growth of blood capillaries in the living mammal
Microscopic observations on the growth of blood capillaries in the living mammal
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DOI：
10.1002/aja.1000640203
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发表时间：
1939-03-01
影响因子：
--
通讯作者：
Clark, EL
中科院分区：
文献类型：
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作者：
Clark, ER;Clark, EL
对活体动物毛细血管的显微研究表明，两栖动物幼虫的透明尾巴和成年兔耳内的透明腔的毛细血管在发芽、吻合、管腔形成和剩余毛细血管缩回等方面的生长过程是相同的。新形成的血管内皮在特异性、精致性和合胞性等方面也具有相似的特点。观察到类似成纤维细胞的细胞粘附在新的哺乳动物毛细血管壁上，并保持为外基质细胞。这一过程与在两栖动物幼虫中观察到的过程相似。未见外层细胞的收缩性。随着血管的发展和血液开始循环，腔室中生长的毛细血管外的组织发生了特征性的变化。纤维蛋白溶解，血管外液体变成软凝胶。随后，成纤维细胞迁移到生长空间，结缔组织在血管间分化。腔室中血管的生长受到空间深度、温度和表皮或软骨的存在的影响。新毛细血管的生长在活跃循环和炎症渗出物存在时发生得最快。有利于新生血管的生长条件也刺激了同一区域其他组织的生长。
Microscopic studies of blood capillaries in the living animal showed that the process of growth in respect to sprout formation, anastomosis, lumen formation and retraction of surplus capillaries was the same in the transparent tails of amphibian larvae and in transparent chambers installed in the ears of adult rabbits. The character of the newly formed blood vascular endothelium as regards its specificity, delicacy and syncytial nature was also similar in the 2 regions. Cells resembling fibroblasts were observed to adhere to the walls of new mammalian capillaries and to remain as adventitial cells. The process was similar to that observed in amphibian larvae. Contractility of adventitial cells was not seen. The tissue outside growing capillaries in the chambers underwent characteristic changes as the vessels advanced and the blood began to circulate. Fibrin dissolved and extravascular fluid changed to a soft gel. Subsequently, fibroblasts migrated into the growth space and connective tissue differentiated between the vessels. The growth of vessels in the chamber was modified by depth of space, temp., and presence of epidermis or cartilage. Growth of new capillaries occurred most rapidly in the presence of an active circulation and an inflammatory exudate. The same growth conditions which favored new blood vessels also stimulated growth of other tissues in the same region.