AGGREGATION OF LEUKOCYTES INDUCED BY THE COMPLEMENT-DERIVED PEPTIDE-C3A AND PEPTIDE-C5A AND BY 3 SYNTHETIC FORMYL-METHIONYL PEPTIDES
AGGREGATION OF LEUKOCYTES INDUCED BY THE COMPLEMENT-DERIVED PEPTIDE-C3A AND PEPTIDE-C5A AND BY 3 SYNTHETIC FORMYL-METHIONYL PEPTIDES
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DOI：
10.1159/000232622
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发表时间：
1980-01-01
期刊：
影响因子：
--
通讯作者：
VOGT, W
中科院分区：
文献类型：
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作者：
DAMERAU, B;GRUNEFELD, E;VOGT, W
高度纯化的猪补体肽 C3a 和 C5a 以及 3 个甲酰基甲硫酰肽可诱导人类白细胞发生剂量依赖性聚集。为了达到该效果，需要Ca2+和Mg2+离子；当它们不存在时，肽会诱导特异性脱敏。人血清白蛋白（1%和2%）减少聚集；同等或什至更高浓度的血浆或血清没有抑制作用。 SH试剂、蛋白酶抑制剂（N-甲苯磺酰-L-赖氨酰-氯甲基酮、N-甲苯磺酰-L-苯丙氨酰-氯甲基酮）和秋水仙碱抑制聚集；细胞松弛素B大大增强了它。在可比条件下的剂量反应研究表明，在与趋化性相似的剂量范围内诱导聚集。体内产生的补体衍生肽可能通过两种方式促进白细胞积聚：首先，使白细胞粘附于内皮（相当于聚集），然后促进向炎症部位迁移。
The highly purified hog complement peptides, C3a and C5a, and 3 formyl-methionyl peptides induced dose-dependent aggregation of human leukocytes. For the effect, Ca2+ and Mg2+ ions were required; in their absence the peptides induced specific desensitization. Human serum albumin (1 and 2%) reduced aggregation; equivalent or even higher concentrations of plasma or serum were not inhibitory. SH reagents, protease inhibitors (N-tosyl-L-lysyl-chloromethyl ketone, N-tosyl-L-phenylalanyl-chloromethyl ketone) and colchicine inhibited aggregation; cytochalasin B greatly enhanced it. Dose-response studies under comparable conditions showed that aggregation is induced in similar dose ranges as chemotaxis. Complement-derived peptides generated in vivo may contribute to leukocyte accumulation in 2 ways: first, by causing adherence of leukocytes to endothelium (equivalent to aggregation), and then by promoting migration to the inflammatory site.