The subiculum is a patchwork of discrete subregions.
The subiculum is a patchwork of discrete subregions.
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DOI：
10.7554/elife.37701
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发表时间：
2018-10-30
期刊：
影响因子：
7.7
通讯作者：
Spruston N
中科院分区：
文献类型：
--
作者：
Cembrowski MS;Wang L;Lemire AL;Copeland M;DiLisio SF;Clements J;Spruston N
在海马体中，下托的经典锥体细胞类型充当主要输出，将海马信号传递到不同的下游区域。越来越多的证据表明，下托锥体细胞群实际上可能由离散的亚类组成。在这里，我们研究了整个小鼠下托锥体细胞异质性的程度和组织原则。使用单细胞 RNA-seq，我们发现下托锥体细胞群可以解构为八个可分离的亚类。这些子类被映射到相邻的空间域上，最终产生复杂的层状和柱状组织，在经典的背腹、近端、远端和浅表-深部轴上具有异质性。我们进一步表明，这些转录组定义的亚类对应于差异蛋白产物，并且可以与特定的投影靶标相关。这项工作将下托锥体细胞的复杂景观解构为空间隔离的子类，这些子类可以在未来的实验中观察、控制和解释。
In the hippocampus, the classical pyramidal cell type of the subiculum acts as a primary output, conveying hippocampal signals to a diverse suite of downstream regions. Accumulating evidence suggests that the subiculum pyramidal cell population may actually be comprised of discrete subclasses. Here, we investigated the extent and organizational principles governing pyramidal cell heterogeneity throughout the mouse subiculum. Using single-cell RNA-seq, we find that the subiculum pyramidal cell population can be deconstructed into eight separable subclasses. These subclasses were mapped onto abutting spatial domains, ultimately producing a complex laminar and columnar organization with heterogeneity across classical dorsal-ventral, proximal-distal, and superficial-deep axes. We further show that these transcriptomically defined subclasses correspond to differential protein products and can be associated with specific projection targets. This work deconstructs the complex landscape of subiculum pyramidal cells into spatially segregated subclasses that may be observed, controlled, and interpreted in future experiments.