Cellular composition and cytoarchitecture of the adult human subventricular zone:: A niche of neural stem cells
Cellular composition and cytoarchitecture of the adult human subventricular zone:: A niche of neural stem cells
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DOI：
10.1002/cne.20798
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发表时间：
2006-01-20
影响因子：
2.5
通讯作者：
Alvarez-Buylla, A
中科院分区：
文献类型：
--
作者：
Quinoñes-Hinojosa, A;Sanai, N;Alvarez-Buylla, A
人类大脑中侧脑室的侧壁在整个成人生活中都含有神经干细胞。我们进行了细胞结构和超微结构的研究，在完整的死后大脑（n = 7），并在死后（n = 42）和术中组织（n = 43）样本的侧壁的人侧脑室。随着厚度和细胞密度的变化，在整个侧脑室壁观察到四层：单层室管膜细胞（I层），细胞减少的间隙（II层），由星形胶质细胞组成的细胞带（111层），以及进入脑实质的过渡区（IV层）。与啮齿动物和非人灵长类动物不同，成年人胶质细胞酸性蛋白（GFAP）+室管膜下区（SVZ）星形胶质细胞与室管膜之间存在细胞间隙。增殖细胞核抗原（PCNA）和M67染色显示，前角SVZ和侧脑室体中第11层中的一些星形胶质细胞以及少数GFAP细胞似乎增殖。然而，与啮齿类动物相比，成年人SVZ似乎缺乏链迁移或大量新形成的年轻神经元。只有在前SVZ中，我们发现了具有迁移形态的细长Tuj1+细胞的例子。我们提供了超微结构标准来识别人类SVZ中的不同细胞类型，包括三种不同类型的星形胶质细胞和H层和Ell层之间的一组移位室管膜细胞。这层的超微结构分析揭示了一个显着的星形胶质细胞和室管膜过程的网络。这项工作提供了成人SVZ组织的基本描述。
The lateral wall of the lateral ventricle in the human brain contains neural stem cells throughout adult life. We conducted a cytoarchitectural and ultrastructural study in complete postmortem brains (n = 7) and in postmortem (n = 42) and intraoperative tissue (n = 43) samples of the lateral walls of the human lateral ventricles. With varying thickness and cell densities, four layers were observed throughout the lateral ventricular wall: a monolayer of ependymal cells (Layer I), a hypocellular gap (Layer II), a ribbon of cells (Layer 111) composed of astrocytes, and a transitional zone (Layer IV) into the brain parenchyma. Unlike rodents and nonhuman primates, adult human glial fibrillary acidic protein (GFAP)+ subventricular zone (SVZ) astrocytes are separated from the ependyma by the hypocellular gap. Some astrocytes as well as a few GFAP-cells in Layer 11 in the SVZ of the anterior horn and the body of the lateral ventricle appear to proliferate based on proliferating cell nuclear antigen (PCNA) and M67 staining. However, compared to rodents, the adult human SVZ appears to be devoid of chain migration or large numbers of newly formed young neurons. It was only in the anterior SVZ that we found examples of elongated Tuj1+ cells with migratory morphology. We provide ultrastructural criteria to identify the different cells types in the human SVZ including three distinct types of astrocytes and a group of displaced ependymal cells between Layers H and Ell. Ultrastructural analysis of this layer revealed a remarkable network of astrocytic and ependymal processes. This work provides a basic description of the organization of the adult human SVZ.