High-Throughput Quantitative Analysis by Desorption Electrospray Ionization Mass Spectrometry
High-Throughput Quantitative Analysis by Desorption Electrospray Ionization Mass Spectrometry
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DOI：
10.1016/j.jasms.2008.10.011
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发表时间：
2009-02-01
影响因子：
3.2
通讯作者：
Ouyang, Zheng
中科院分区：
文献类型：
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作者：
Manicke, Nicholas E.;Kistler, Thomas;Ouyang, Zheng
新开发的高通量解吸电喷雾电离 (DEST) 源在定量分析中的性能进行了表征。单次分析包含 96 个样品的阵列，其中包含各种基质中的药物，总分析时间为 3 分钟。这些溶液相样品是通过打印在玻璃上的疏水性 PTFE 墨水进行检查的。对定量准确度、精密度和检测限 (LOD) 进行了表征。对以 PRN-d(7) 作为内标 (IS) 的普萘洛尔 (PRN) 和以 CBZ-d(10) 作为内标的卡马西平 (CBZ) 的无化学背景样品进行了检查。另外两个由不同浓度的 PRN/PRN-d(7) 组成的样品组也是如此，在 10% 尿液或猪脑总脂质提取物的生物环境中，总脂质浓度为 250 ng/mu L。无背景样品在 1.5 秒/样品的总分析时间内检测，显示出良好的定量准确性和精密度，相对误差 (RE) 和相对标准偏差 (RSD) 一般分别小于 3% 和 5%。尿液中的样品和脂质提取物需要较长的分析时间（2.5 秒/样品），并且尿液中的样品的 RSD 值约为 1.0%，脂质提取物样品的 RSD 值为 4%，两组的 RE 值均小于 3%。在没有化学背景的情况下进行分析时，PRN 和 CBZ 的 LOD 分别为 10 和 30 fmol。在 10% 尿液中分析时，PRN 的 LOD 增加至 400 fmol，在脑脂质提取物中分析时增加至 200 fmol。 (J Am Soc Mass Spectrom 2009, 20, 321-325) (C) 2009 由 Elsevier Inc. 代表美国质谱学会出版
A newly developed high-throughput desorption electrospray ionization (DEST) Source was characterized in tern-is of its performance in quantitative analysis. A 96-sample array, containing pharmaceuticals in various matrices, was analyzed in a single run with a total analysis time of 3 min. These solution-phase samples were examined from a hydrophobic PTFE ink printed on glass. The quantitative accuracy, precision, and limit of detection (LOD) were characterized. Chemical background-free samples of propranolol (PRN) with PRN-d(7) as internal standard (IS) and carbamazepine (CBZ) with CBZ-d(10) as IS were examined. So were two other sample sets consisting of PRN/PRN-d(7) at varying concentration in a biological milieu of 10% urine or porcine brain total lipid extract, total lipid concentration 250 ng/mu L.The background-free samples, examined in a total analysis time of 1.5 s/sample, showed good quantitative accuracy and precision, with a relative error (RE) and relative standard deviation (RSD) generally less than 3% and 5% respectively. The samples in urine and the lipid extract required a longer analysis time (2.5 s/sample) and showed RSD Values of around 1.0% for the samples in urine and 4%, for the lipid extract samples and RE values of less than 3% for both sets. The LOD for PRN and CBZ when analyzed without chemical background was 10 and 30 fmol, respectively. The LOD of PRN increased to 400 fmol analyzed in 10% urine, and 200 fmol when analyzed in the brain lipid extract. (J Am Soc Mass Spectrom 2009, 20, 321-325) (C) 2009 Published by Elsevier Inc. on behalf of American Society for Mass Spectrometry