Telomere length in subpopulations of human hematopoietic cells
Telomere length in subpopulations of human hematopoietic cells
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DOI：
10.1634/stemcells.21-6-654
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发表时间：
2003-01-01
期刊：
影响因子：
5.2
通讯作者：
Lansdorp, PM
中科院分区：
文献类型：
--
作者：
Van Ziffle, JAG;Baerlocher, GM;Lansdorp, PM
为了检验人类造血细胞中端粒长度与其增殖潜力相关的假设，我们分析了高度纯化的骨髓细胞亚群中的端粒长度。基于CD 34和CD 38细胞表面标志物分选细胞，并基于Hoechst 33342染料流出额外分选两个样品，从而分离侧群（SP）细胞。使用新开发的荧光原位杂交（flow-FISH）方法分析有限数量的分选细胞中的端粒长度，其中相对于同一管中的对照细胞测量感兴趣的细胞中的端粒探针的杂交。在分析的所有7个骨髓样品中，来自同一供体的CD 34（+）CD 38（-）细胞中的端粒长度长于CD 34（+）CD 38（+）细胞中的端粒长度（p < 0.02）。分选的SP细胞的结果不太清楚：这些细胞中的端粒荧光非常不均匀，并且不能观察到端粒长度相对于CD 34（+）CD 38（-）细胞的可重复差异。我们的结论是，端粒长度在造血细胞亚群似乎是与已知的增殖潜力，这样的细胞和端粒长度的基础上，细胞的进一步表征是必要的非常罕见的造血和其他组织的前体富集。
In order to test the hypothesis that the telomere length in human hematopoietic cells correlates with their proliferative potential, we analyzed the telomere length in highly purified subpopulations of bone marrow cells. Cells were sorted on the basis of CD34 and CD38 cell surface markers, and two samples were additionally sorted on the basis of Hoechst 33342 dye efflux allowing isolation of side population (SP) cells. The telomere length in limiting numbers of sorted cells was analyzed using a newly developed fluorescence in situ hybridization (flow-FISH) method in which hybridization of telomere probe in cells of interest is measured relative to control cells in the same tube. In all seven bone marrow samples analyzed, the telomere length in CD34(+)CD38(-) cells was longer than in CD34(+)CD38(+) cells from the same donor (p < 0.02). Results with sorted SP cells were less clear: the telomere fluorescence in these cells was very heterogeneous, and a reproducible difference in telomere length relative to CD34(+)CD38(-) cells could not be observed. We conclude that the telomere length in subpopulations of hematopoietic cells does appear to be correlated with the known proliferative potential of such cells and that further characterization of cells on the basis of telomere length is warranted for enrichment of very rare precursors of hematopoietic and other tissues.