IN-VITRO CULTIVATION OF HUMAN TUMORS - ESTABLISHMENT OF CELL LINES DERIVED FROM A SERIES OF SOLID TUMORS
IN-VITRO CULTIVATION OF HUMAN TUMORS - ESTABLISHMENT OF CELL LINES DERIVED FROM A SERIES OF SOLID TUMORS
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DOI：
10.1093/jnci/51.5.1417
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发表时间：
1973-01-01
期刊：
影响因子：
--
通讯作者：
PARKS, WP
中科院分区：
文献类型：
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作者：
GIARD, DJ;AARONSON, SA;PARKS, WP
为了建立细胞系，在体外培养了一系列200个人类肿瘤细胞。用外植体和胰酶消化技术，从包括癌症、肉瘤、黑色素瘤和脑瘤在内的13种肿瘤中建立了细胞系。所有这些细胞系在培养超过1年后，都表现出显著的屈光性、多层和交叉，在形态上与正常接触抑制的人成纤维细胞或上皮细胞系不同。它们还在正常细胞的单层上形成集落，并在软琼脂中高效生长。初步结果显示，所有受试小鼠的染色体都出现异常，在抗胸腺血清处理的小鼠中，9个细胞系中有8个形成了肿瘤。来自随机肿瘤材料的细胞系的建立率(约6%)表明，具有转化细胞特性的细胞可以从肿瘤组织中恢复，但它也强调了在这一领域改进方法学的必要性。
A series of 200 human tumors were cultivated in vitro in an attempt to establish cell lines. Lines were established, with explant and trypsinization techniques, from 13 tumors including carcinomas, sarcomas, melanomas, and brain tumors. All these lines, In culture for over 1 year, exhibited marked refractility, multilayering, and criss-crossing and were morphologically distinct from normal contact-inhibited human fibroblast or epithelial lines. They also formed colonies on monolayers of normal cells and grew with a high efficiency in soft agar. Preliminary results indicated abnormal chromosomal patterns in all lines tested, and 8 of 9 cell lines formed tumors in antithymo~yte serum-treated mice. The rate of establishment (approximately 6%) of lines from random neoplastic material demonstrated that cells with properties of transformed cells could be recovered from tumor tissue, but it also emphasized the need for improved methodology in this area.