Dynamic changes in the rate of amylase release induced by various secretagogues examined in isolated rat parotid cells by using column perifusion.
Dynamic changes in the rate of amylase release induced by various secretagogues examined in isolated rat parotid cells by using column perifusion.
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通过使用柱灌注在分离的大鼠腮腺细胞中检查各种促分泌剂诱导的淀粉酶释放速率的动态变化。
DOI：
10.2170/jjphysiol.41.443
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
E. Nezu
中科院分区：
文献类型：
--
作者：
K. Yoshimura;E. Nezu
分离的腮腺腺泡细胞灌流在小圆柱包埋在生物凝胶P-2珠作为一种惰性的支持矩阵，和氨基甲酰胆碱，P物质，和异丙肾上腺素对淀粉酶释放率的影响进行了检查，通过测量流出物中的淀粉酶活性。连续刺激氨甲酰胆碱和P物质的淀粉酶释放是双相的。它们引起淀粉酶释放速率的快速和大幅增加，在刺激开始后30至60秒达到最大值，随后迅速下降至较低的持续水平，只要存在激动剂，该水平就保持不变。淀粉酶释放速率的迅速下降是由于耐火度的迅速发展。重复1分钟的脉冲刺激，这些促分泌素表明，恢复不应期也迅速发病，和1分钟的冲洗足以引起显着恢复不应期为氨甲酰胆碱和P物质。恢复，但是，不完全冲洗10分钟后。另一方面，通过用异丙肾上腺素连续刺激的淀粉酶释放发展得更慢，在刺激开始后约6分钟达到峰值速率。在异丙肾上腺素的作用中未观察到不应性。氨甲酰胆碱或P物质对淀粉酶释放速率的最大效应高于异丙肾上腺素。这些结果表明，氨基甲酰胆碱和P物质对淀粉酶释放的明显小的影响，早期报告使用分批系统可能是由于快速发展的耐火这些促分泌素，但不是异丙肾上腺素。
Isolated parotid acinar cells were perifused in small columns by embedding them in Bio-Gel P-2 beads as an inert supporting matrix, and the effect of carbamylcholine, substance P, and isoproterenol on the rate of amylase release was examined by measuring amylase activity in the effluent. Amylase release by continuous stimulation with carbamylcholine and substance P was biphasic. They caused a rapid and large increase in the rate of amylase release that reached maximum 30 to 60s after the onset of stimulation, followed by a rapid decline to a lower sustained level that was maintained as long as the agonists were present. The rapid decline in the rate of amylase release was due to rapid development of refractoriness. Repeated 1 min pulse stimulation with these secretagogues showed that recovery from refractoriness was also rapid in onset, and 1 min of washout was sufficient to cause significant recovery from refractoriness for both carbamylcholine and substance P. Recovery, however, was not complete after 10 min of washout. Amylase release by continuous stimulation with isoproterenol, on the other hand, developed more slowly with the peak rate being attained at about 6 min after the onset of stimulation. Refractoriness was not observed in the effect of isoproterenol. The maximum effect in the rate of amylase release attained by carbamylcholine or substance P was higher than that by isoproterenol. These results suggest that the apparent small effect of carbamylcholine and substance P on amylase release reported earlier by using batch systems is probably due to the rapid development of refractoriness to these secretagogues, but not to isoproterenol.