Next-generation and further transgenerational effects of bisphenol A on zebrafish reproductive tissues.
Next-generation and further transgenerational effects of bisphenol A on zebrafish reproductive tissues.
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DOI：
10.1016/j.heliyon.2018.e00788
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发表时间：
2018-09
期刊：
影响因子：
4
通讯作者：
Tokumoto T
中科院分区：
文献类型：
--
作者：
Akhter A;Rahaman M;Suzuki RT;Murono Y;Tokumoto T
在斑马鱼中研究了内分泌干扰物双酚 A (BPA) 的下一代效应和进一步的跨代效应。通过饮食对雄性和雌性斑马鱼进行 BPA 治疗对生殖因素（如性腺活性、生育力、孵化率和后代畸形）的影响进行了研究，直至第三代（F3）。初代(F0)的BPA处理不仅导致卵巢和睾丸回缩，而且降低子代的存活率，增加子代的畸形率。尽管经过处理的鱼幸存的第一代（F1）后代的整体表型最初看起来是正常的，但我们在它们成熟到成年后发现它们的生殖组织出现异常。尽管幼鱼接受正常饮食，但 40% 的 F1 代鱼的卵巢仍然很小，并且没有发育出卵黄卵母细胞。此外，不育雄性鱼的比例（48%）高于对照（10%）。还观察到对第二代（F2）和F3代鱼的繁殖力产生不利的跨代影响。在每一代中，经BPA处理的祖先的胚胎存活率均显着降低，并且出现异常胚胎。这些结果表明，BPA 的影响不仅通过卵母细胞而且还通过精子传递给后代。
Next-generation effects and further transgenerational effects of an endocrine disruptor, bisphenol A (BPA), were investigated in zebrafish. The effects of BPA treatment through dietary administration in male and female zebrafish on reproductive factors, such as gonadal activity, fertility, hatching rate and malformation in subsequent generations, were examined through the third filial (F3) generation. BPA treatment of initial generation (F0) not only caused retraction of the ovaries and testes but also lowered the survival rate and increased the rate of malformation of the offspring. Although the overall phenotypes of the surviving first filial (F1) generation offspring of treated fish initially appeared to be normal, we found abnormalities in their reproductive tissues after they matured to adulthood. Although the juveniles were fed a normal diet, the ovaries of 40% of the F1 generation fish remained small and did not develop vitellogenic oocytes. Moreover, sterile male fish appeared at a higher percentage (48%) than control (10%). Adverse transgenerational effects on the fecundity of the second filial (F2) and F3 generation fish were also observed. In each generation, survival rate of embryos were significantly low and abnormal embryos were appeared in offspring from BPA treated ancestral. These results demonstrate that the effects of BPA are transferred to subsequent generations not only through oocytes but also through sperm.