Effect of metallic nanoparticles on amyloid fibrils and their influence to neural cell toxicity
Effect of metallic nanoparticles on amyloid fibrils and their influence to neural cell toxicity
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DOI：
10.1007/s12274-020-2748-2
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发表时间：
2020-04-14
期刊：
影响因子：
9.9
通讯作者：
Bystrenova, Eva
中科院分区：
文献类型：
--
作者：
Barbalinardo, Marianna;Antosova, Andrea;Bystrenova, Eva
通过外源纳米材料修饰淀粉样原纤维的细胞毒性对于了解控制蛋白质聚集在相关疾病中的作用的过程至关重要。由于纳米颗粒尺寸小且表面积与体积比高，纳米颗粒对淀粉样蛋白稳定性的影响引起了人们极大的兴趣。各种物理化学参数在溶液中蛋白质和纳米粒子的相互作用中发挥作用，从而影响预形成原纤维的解聚。我们采用多种技术方法研究了两种金属纳米颗粒对溶菌酶淀粉样原纤维的影响，并重点关注它们对人神经母细胞瘤细胞（SH-SY5Y）细胞毒性的影响。特别是开展了荧光、红外和圆二色光谱、光学和原子力显微镜实验；对结果进行分析，以合理化这些复合物对神经细胞活力的影响。值得注意的是，与单独的原纤维或含 AgNP 的原纤维不同，AuNP 存在下的原纤维即使在高浓度下也不会产生显着的细胞毒性作用，并且淀粉样蛋白降解作用是可见的。
The modification of amyloid fibrils cytotoxicity through exogenous nanomaterials is crucial to understand the processes controlling the role of protein aggregation in the related diseases. The influence of nanoparticles on amyloid stability yields great interest due to the small size and high surface area-to-volume ratio of nanoparticles. Various physico-chemical parameters play a role in the interaction of proteins and nanoparticles in solution, thus influencing the disaggregation of preformed fibrils. We have examined the influence of two kinds of metallic nanoparticles on lysozyme amyloid fibrils using a multi-technique approach and focalized their impact on cytotoxicity on human neuroblastoma cells (SH-SY5Y). In particular, fluorescence, infrared and circular dichroism spectroscopies, optical and atomic force microscopy experiments have been carried out; the results are analyzed to rationalize the effects of these complexes on neural cell viability. It is remarkable, that the fibrils in the presence of AuNPs, unlike fibrils alone or with AgNPs, do not generate a significant cytotoxic effect even at high concentration and an amyloid degradation effect is visible.