Functionally graded bioactive coatings: reproducibility and stability of the coating under cell culture conditions.
Functionally graded bioactive coatings: reproducibility and stability of the coating under cell culture conditions.
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DOI：
10.1016/j.actbio.2005.12.003
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发表时间：
2006-03
影响因子：
9.7
通讯作者：
S. Foppiano;S. Marshall;E. Saiz;A. Tomsia;G. Marshall
中科院分区：
文献类型：
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作者：
S. Foppiano;S. Marshall;E. Saiz;A. Tomsia;G. Marshall
这项工作旨在提供一个基本的协议，处理功能梯度生物活性玻璃涂层（FGC），可靠地粘附到钛合金（Ti6 Al 4V）之前，在体内评价。评估了制造工艺对玻璃结构和涂层性能的再现性的影响，以及细胞培养条件对涂层完整性的影响。将FGC的结构与用作顶部涂层的铸态玻璃的结构进行比较。X射线衍射（XRD）表明，制备过程中产生5.9±3.0vol.%结晶，而玻璃作为铸造无定形。在模拟体液（SBF）中，铸态玻璃和涂层表现相似：形成富含磷酸盐的非晶层，并且其在4周内结晶成磷灰石样矿物（傅立叶变换红外光谱（FTIR）、XRD、扫描电子显微镜（SEM））。通过测量厚度和结晶度，从三批涂层测试制造过程的再现性。培养MC 3 T3-E1.4小鼠前成骨细胞，并诱导其在FGC上矿化，无论是制备的还是在SBF中预处理的。FGC中的亚表面玻璃二氧化硅网络受到细胞培养条件的损害。在预处理过程中形成结晶磷酸盐（XRD、FTIR和SEM）。SBF预处理使涂层稳定。因此，建议在SBF中孵育以产生稳定的涂层。
This work sought to provide a basic protocol for treatment of functionally graded bioactive glass coatings (FGC) that reliably adhere to titanium alloy (Ti6Al4V) prior to in vivo evaluation. The effect of the fabrication process on glass structure and reproducibility of the coating’s properties, and the effect of cell culture conditions on the integrity of the coating were assessed. The structure of FGCs was compared to that of the as cast glass used as a top coating. X-ray diffraction (XRD) showed that the fabrication process resulted in 5.9±3.0vol.% crystallization, while glass as cast was amorphous. Glass as cast and coatings behaved similarly in simulated body fluid (SBF): an amorphous layer rich in phosphate formed, and it crystallized, over 4weeks, into apatite-like mineral (Fourier transform infrared spectroscopy (FTIR), XRD, scanning electron microscopy (SEM)). Reproducibility of the fabrication process was tested from three batches of coatings by measuring thickness and crystallinity. MC3T3-E1.4 mouse pre-osteoblast cells were cultured and induced to mineralize on FGCs, either as made or pre-conditioned in SBF. The sub-surface glass silica network in FGCs was compromised by cell culture conditions. A crystalline phosphate was formed during pre-conditioning (XRD, FTIR, and SEM). SBF-pre-conditioning stabilized the coatings. Thus incubation in SBF is recommended to produce a stable coating.