Mucociliary Clearance in Mice Measured by Tracking Trans-tracheal Fluorescence of Nasally Aerosolized Beads.
Mucociliary Clearance in Mice Measured by Tracking Trans-tracheal Fluorescence of Nasally Aerosolized Beads.
复制标题
通过跟踪鼻雾化珠的经气管荧光测量小鼠的粘膜纤毛清除率。
DOI：
10.1038/s41598-018-33053-2
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发表时间：
2018
影响因子：
4.6
通讯作者：
Grubb,BarbaraR
中科院分区：
文献类型：
--
作者：
Rogers,TroyD;Ostrowski,LawrenceE;Livraghi-Butrico,Alessandra;Button,Brian;Grubb,BarbaraR
粘膜纤毛清除（MCC）是清除气道的第一道防线。在基因工程小鼠中，可以分别研究该系统的每个组分（纤毛搏动、粘液、气道表面水合）以确定其对MCC的贡献。由于MCC难以在小鼠中测量，因此这些研究中通常省略MCC测量。我们报告了一种简单的方法来测量MCC在小鼠鼻吸入雾化荧光珠和经气管珠跟踪。该方法与现有方法相比具有许多优点：（1）沉积小体积的液体，因此最小程度地干扰气道表面;（2）气道上的珠行为可以可视化;（3）对成年或新生小鼠有用;（4）设备相对便宜且容易获得。麻醉剂的种类对MCC的发生率没有显著影响，但用磁珠使气道过载可显著降低MCC。此外，活小鼠（3.11 mm/min）与最近安乐死小鼠（3.10 mm/min）的微珠转运速率无差异。在含有UTP的溶液中雾化5分钟的珠粒显著增加了MCC的速率，表明我们的方法在测试各种药理学试剂对MCC的作用中具有价值。
Mucociliary clearance (MCC) is the first line of defense in clearing airways. In genetically engineered mice, each component of this system (ciliary beat, mucus, airway surface hydration) can be studied separately to determine its contribution to MCC. Because MCC is difficult to measure in mice, MCC measurements are often omitted from these studies. We report a simple method to measure MCC in mice involving nasal inhalation of aerosolized fluorescent beads and trans-tracheal bead tracking. This method has a number of advantages over existing methods: (1) a small volume of liquid is deposited thus minimally disturbing the airway surface; (2) bead behavior on airways can be visualized; (3) useful for adult or neonatal mice; (4) the equipment is relatively inexpensive and easily obtainable. The type of anesthetic had no significant effect on the rate of MCC, but overloading the airways with beads significantly decreased MCC. In addition, the rate of bead transport was not different in alive (3.11 mm/min) vs recently euthanized mice (3.10 mm/min). A 5-min aerosolization of beads in a solution containing UTP significantly increased the rate of MCC, demonstrating that our method would be of value in testing the role of various pharmacological agents on MCC.