Effects of aortic coarctation on aortic antioxidant enzymes and NADPH oxidase protein expression
Effects of aortic coarctation on aortic antioxidant enzymes and NADPH oxidase protein expression
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DOI：
10.1016/j.lfs.2004.10.014
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发表时间：
2005-01-07
期刊：
影响因子：
6.1
通讯作者：
Vaziri, ND
中科院分区：
文献类型：
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作者：
Sindhu, RK;Roberts, CK;Vaziri, ND
肾动脉上方的腹主动脉缩窄导致狭窄部位上方的严重高血压，并为同时测试同一动物的压力升高和降低以及随后的切应力的影响提供了一个模型。压力增加的影响。本身就是。关于氧化应激和抗氧化物酶的表达尚不清楚。我们研究了术后4周假手术对照组和主动脉缩窄组大鼠主动脉狭窄处上下段抗氧化酶和NADPH氧化酶(gp91(Phox)亚单位)的蛋白表达。与对照组比较。环扎组大鼠胸主动脉NADPH氧化酶、过氧化氢酶(CAT)、铜/锌超氧化物歧化酶(SOD)和锰-超氧化物歧化酶(Mn-SOD)蛋白含量显著升高。而锰超氧化物歧化酶则相反。在血压不升高的腹主动脉狭窄段下，铜/锌超氧化物歧化酶和NADPH氧化酶蛋白丰度无明显变化，而CAT蛋白丰度在腹主动脉也有升高。胸主动脉段和腹主动脉段的谷胱甘肽过氧化物酶(GPX)蛋白含量均无明显变化。缩窄性高血压与主动脉CAT升高相关。铜/锌超氧化物歧化酶。锰超氧化物歧化酶和NADPH氧化酶蛋白表达。NADPH氧化酶的上调增加了本研究中提到的活性氧物种(ROS)的产生，并有助于灭活一氧化氮(NO)，如先前在该模型中所示。抗氧化酶的上调可能是面对压力升高和氧化应激时的一种代偿反应。在血压不升高的腹主动脉中，蛋白质丰度的正常表明，压力机械因素的作用，而不是两个节段相似的循环体液因素，作为导致抗氧化酶表达增加的机制。(C)2004 Elsevier Inc.保留所有权利。
Abdominal aortic coarctation above the renal arteries leads to severe hypertension above the stenotic site and provides a model for simultaneous testing of the effects of increased and decreased pressure and consequently shear stress in the same animal. The effects of increased pressure. per se. on oxidative stress and antioxidant enzyme expression is unknown. We studied the protein expressions of antioxidant enzymes and NADPH oxidase (gp91(phox) subunit) in the aortic segments above and below the stenosis site in sham-operated control and aortic-banded rats at four weeks postoperatively. Compared with the control group. the banded group showed significant up-regulation of NADPH oxidase, catalase (CAT), Cu/Zn superoxide dismutase (SOD) and Mn SOD protein content in the thoracic aorta. In contrast, Mn SOD. Cu/Zn SOD and NADPH oxidase protein abundance were unchanged in the abdominal aortic segment below the stricture where blood pressure is not elevated, whereas CAT protein abundance was also elevated in the abdominal aorta. No changes were noted for glutathione peroxidase (GPX) protein content either in the thoracic or abdominal aortic segments. Coarctation-induced hypertension is associated with increased aortic CAT. Cu/Zn SOD. Mn SOD and NADPH oxidase protein expression. The up-regulation of NADPH oxidase increases reactive oxygen species (ROS) generation noted in the present study and contributes to inactivation of nitric oxide (NO) as shown previously in this model. Upregulation of antioxidant enzymes may be a compensatory response in the face of elevated pressure and oxidative stress. The normality of protein abundance in the abdominal aorta wherein blood pressure is not elevated points to the role of baromechanical factors, as opposed to circulating humoral factors that were similar in both segments, as a mechanism responsible for increased antioxidant enzyme expression. (C) 2004 Elsevier Inc. All rights reserved.