Monoamine oxidase inhibitory components from the roots of Sophora flavescens
Monoamine oxidase inhibitory components from the roots of Sophora flavescens
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DOI：
10.1007/bf02977714
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发表时间：
2005-02-01
影响因子：
6.7
通讯作者：
Ro, JS
中科院分区：
文献类型：
--
作者：
Hwang, JS;Lee, SA;Ro, JS
在我们从天然资源中寻找单胺氧化酶（MAO）抑制剂的过程中，我们发现苦参根的甲醇提取物对小鼠脑单胺氧化酶（MAO）表现出抑制作用。提取物的生物活性导向分离产生了两种已知的黄酮类化合物，芒柄花素（1）和苦参素F（2），作为活性化合物沿着三种非活性化合物，氧化苦参碱（3），三叶豆苷（4）和β-谷甾醇（5）。芒柄花素（1）和苦参素F（2）对单胺氧化酶（MAO）的抑制作用呈剂量依赖性，IC_（50）分别为13.2和69.9 μ M。芒柄花素（1）对单胺氧化酶-B的抑制作用（IC_（50）：11.0 μ M）略强于单胺氧化酶-A（IC_（50）：21.2 μ M）。苦参素F（2）也优先抑制MAO-B活性而不是MAO-A活性，IC_（50）值分别为63.1和103.7 μ M。
In our search for monoamine oxidase (MAO) inhibitors from natural resources, we found that the methanol extract of the roots of Sophora flavescens showed an inhibitory effect on mouse brain monoamine oxidase (MAO). Bioactivity-guided isolation of the extract yielded two known flavonoids, formononetin (1) and kushenol F (2), as active compounds along with three inactive compounds, oxymatrine (3), trifolirhizin (4), and beta-sitosterol (5). Formononetin (1) and kushenol F (2) showed significant inhibitory effects on MAO in a dose-dependent manner with IC50 values of 13.2 and 69.9 muM, respectively Formononetin (1) showed a slightly more potent inhibitory effect against MAO-B (IC50: 11.0 muM) than MAO-A (IC50: 21.2 muM). Kushenol F (2) also preferentially inhibited the MAO-B activity than MAO-A activity with the IC50 values of 63.1 and 103.7 muM, respectively.