Functional analysis of the Escherichia coli zinc transporter ZitB
Functional analysis of the Escherichia coli zinc transporter ZitB
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DOI：
10.1111/j.1574-6968.2002.tb11402.x
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发表时间：
2002-10-08
影响因子：
2.1
通讯作者：
Rensing, C
中科院分区：
文献类型：
--
作者：
Lee, SM;Grass, G;Rensing, C
通过定点突变研究了大肠杆菌中负责Zn（II）外排的膜转运蛋白ZitB，以阐明单个氨基酸残基的功能。位于预测的跨膜结构域中的几个带电或极性残基H53、H159、D163和D186的取代导致ZitB功能的丧失。与此相反，无论是氨基末端或羧基末端的区域，都富含组氨酸，是必需的功能。(C)2002年，欧洲微生物学会联合会。由Elsevier Science B.V.出版，版权所有。
The membrane transporter ZitB responsible for Zn(II) efflux in Escherichia coli was studied by site-directed mutagenesis to elucidate the function of individual amino acid residues. Substitutions of several charged or polar residues, H53, H159, D163 and D186, located in predicted transmembrane domains resulted in loss of ZitB function. In contrast, neither the amino-terminal nor the carboxy-terminal regions, both histidine-rich, were required for function. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.