Dysbiosis of the gut microbiome impairs mouse skeletal muscle adaptation to exercise.
Dysbiosis of the gut microbiome impairs mouse skeletal muscle adaptation to exercise.
复制标题
DOI：
10.1113/jp281788
复制
发表时间：
2021-11
期刊：
影响因子：
--
通讯作者：
McCarthy JJ
中科院分区：
文献类型：
--
作者：
Valentino TR;Vechetti IJ Jr;Mobley CB;Dungan CM;Golden L;Goh J;McCarthy JJ
有新的证据表明肠道微生物群--骨骼肌轴。这项研究的目的是确定完整的肠道微生物群是否对骨骼肌适应运动是必要的。将42只4月龄雌性C57BL/6J小鼠随机分为未处理组(U组)、抗生素处理组(T组)、非跑步对照组(CU组或CT组)、递进式加权车轮跑组(POWER组，P组)、未处理组(PU组)和抗生素处理组(PT组)。抗生素治疗导致肠道微生物群的破坏，CT组和PT组的肠道微生物群细菌种类都明显减少。PU组和PT组之间的训练刺激相同，分别为每周跑步活动(12.35±2.06vs.11.09±1.76公里)和总跑步活动(778.9±130.5 vs.703.8±112.9公里)。与PU相比，PT的比目鱼肌1型和2a型纤维以及足底2b/x型纤维的肥大程度较轻。PT组大鼠动力后PU足底肌肌卫星细胞和肌核丰度无明显变化。动力后PU足底肌纤维型向氧化程度更高的2a型纤维成分的转变在PT中被钝化。血清细胞因子水平在所有组之间没有差异，这表明肠道微生物群的破坏不会导致全身炎症。这项研究的结果提供了第一个证据，表明完整的肠道微生物群是骨骼肌适应运动所必需的。成年雌性小鼠连续给予抗生素诱导肠道菌群失调，然后进行递增重量轮式跑(功率)8周。在对照组中，未经治疗的小鼠，电力导致比目鱼肌和足底肌肥大和纤维型移位，这在生物失调的小鼠中显著减少。这些发现表明，骨骼肌完全适应运动需要一个健康的肠道微生物群。
There is emerging evidence of a gut microbiome–skeletal muscle axis. The purpose of this study was to determine if an intact gut microbiome was necessary for skeletal muscle adaptation to exercise. Forty-two 4-month-old female C57BL/6J mice were randomly assigned to untreated (U) or antibiotic-treated (T) non-running controls (CU or CT, respectively) or progressive weighted wheel running (PoWeR, P) untreated (PU) or antibiotic-treated (PT) groups. Antibiotic treatment resulted in disruption of the gut microbiome as indicated by a significant depletion of gut microbiome bacterial species in both CT and PT groups. The training stimulus was the same between PU and PT groups as assessed by weekly (12.35 ± 2.06 vs. 11.09 ± 1.76 km/week, respectively) and total (778.9 ± 130.5 vs. 703.8 ± 112.9 km, respectively) running activity. In response to PoWeR, PT showed less hypertrophy of soleus type 1 and 2a fibres and plantaris type 2b/x fibres compared to PU. The higher satellite cell and myonuclei abundance of PU plantaris muscle after PoWeR was not observed in PT. The fibre-type shift of PU plantaris muscle to a more oxidative type 2a fibre composition following PoWeR was blunted in PT. There was no difference in serum cytokine levels among all groups suggesting disruption of the gut microbiome did not induce systemic inflammation. The results of this study provide the first evidence that an intact gut microbiome is necessary for skeletal muscle adaptation to exercise. Adult female mice were continuously treated with antibiotics to induce dysbiosis of the gut microbiome and then underwent progressive weighted wheel running (PoWeR) for 8 weeks. In control, untreated mice, PoWeR induced hypertrophy and a fiber-type shift in both the soleus and plantaris muscles that was significantly diminished in mice with dysbiosis. These findings suggest a healthy gut microbiome is required for skeletal muscle to fully adapt to exercise.